纳米Fe_3O_4/C225复合物对结直肠癌LoVo细胞放射增敏作用的初步研究  

A preliminary study on radiosensitization effect of nano Fe_3O_4/C225 complex on human colorectal cancer LoVo cells

作　　者：孟庆红[1] 孙新臣 孙苏平[1] 于静萍[1] 倪新初[1] 赵迪[1] 

机构地区：[1]南京医科大学附属常州第二人民医院放疗科,江苏常州213003 [2]南京医科大学第一附属医院 [3]江苏省人民医院放疗科,210009

出　　处：《临床肿瘤学杂志》2014年第7期584-588,共5页Chinese Clinical Oncology

摘　　要：目的：探讨纳米Fe3 O4/C225复合物（简称复合物）对结直肠癌LoVo细胞放射增敏性的影响。方法采用MTT法观察不同浓度复合物（0、3.125、6.25、12.5、25、50、100mg/L）作用24h后的增殖抑制率，根据实验设计分为单纯照射组、C225＋照射组及复合物＋照射组，采用克隆形成实验检测3组经0、2、4、6、8和10Gy X线照射后的存活分数（ SF），流式细胞仪检测3组经4Gy X线照射24h后的细胞周期情况，采用Western blotting检测经4Gy X线照射24h后的表皮生长因子受体（ EGFR）蛋白水平及其相关信号通路中p-P38和p-Akt蛋白水平。结果在3.125～100mg/L范围内，随复合物浓度增加， LoVo细胞的增殖抑制率升高，0、3.125、6.25、12.5、25、50、100mg/L的增殖抑制率依次为0％、（8.950±0.086）％、（14.760±0.011）％、（29.860±0.001）％、（41.750±0.017）％、（59.770±0.010）％和（78.710±0.017）％，组间差异有统计学意义（P＜0.05）；复合物＋照射组的SF、S期细胞比例及p-Akt、EGFR蛋白水平均低于C225＋照射组和单纯照射组，G0/G1期、G2/M期细胞比例及p-P38蛋白水平均高于C225＋照射组和单纯照射组，以上差异均有统计学意义（ P＜0.05）。结论纳米Fe3 O4/C225复合物可抑制LoVo细胞增殖并具有放射增敏作用，可能与G0/G1期阻滞及EGFR相关信号通路受抑制有关。Objective To investigate the radiosensitivity enhancement and possible mechanism of nano Fe3 O4/C225 complex on human colorectal cancer LoVo cells. Methods In vitro inhibition rates of cell proliferation at 24h after treatment with different con-centrations （0, 3.125, 6.25, 12.5, 25, 50, 100 mg/mL） of nano Fe3O4/C225 complex were determined by MTT. According to the experimental protocol, the below experiments were carried out in irradiation group, C225＋irradiation group and complex＋irradiation group. The colony formation assay was employed to observe the surviving fraction （ SF） of three groups after X-ray irradiation of 0, 2, 4, 6, 8, 10Gy. The cell cycle of three groups at 24h after X-ray irradiation of 4Gy was measured by flow cytometry. Western blotting was employed to measure the protein level of epidermal growth factor receptor （ EGFR） and p-P38, p-Akt in the related of signaling pathway. Results The proliferation inhibition rate of LoVo cells gradually increased with the increasing concentration of Fe3 O4/C225 complex ranging from 3.125 to 100 mg/L. The proliferation inhibition rates were 0%, （8.950± 0.086）%, （14.760± 0.011）%, （29.860± 0.001）%, （41.750±0.017）%, （59.770±0.010）% and （78.710± 0.017）% at the concentrations of 0, 3.125, 6.25, 12.5, 25, 50, 100mg/mL with statistically significant differences （ P〈0.05） . Compared with irradiation group and C225＋irradiation group, there were lower SF, percentage of S cells and protein levels of p-Akt, EGFR and higher percentages of G0/G1 and G2/M and protein level of p-P38 in complex＋irradiation group with statistically significant differences （P〈0.05）. Conclusion Nano Fe3O4/C225 complex could inhibit the proliferation of LoVo cells with radiosensitizing effect possibly via the arrest at the G0/G1 phase and in-hibition of EGFR signaling pathways.

关 键 词：纳米Fe3 O4 C225复合物 人结直肠癌 放疗增敏 表皮生长因子受体 

分 类 号：R735.34[医药卫生—肿瘤]