白介素-7对小鼠肝纤维化模型肝组织病理的影响  

Influence of interleukin-7 on hepatic pathology in mice with liver fibrosis

作　　者：刘佩佩[1] 刘翠芸[1] 付文娟[1] 余东山[1] 陈明发[1] 罗杰[1] 姚雪兵[1] 席文娜[1] 高珍[1] 孙水林[1] 

机构地区：[1]南昌大学第二附属医院感染性疾病科,江西省南昌市330006

出　　处：《世界华人消化杂志》2014年第21期3077-3082,共6页World Chinese Journal of Digestology

基　　金：江西省教育厅科技支撑计划基金资助项目;No.GJJ13164;江西省卫生厅基金资助项目;No.20131068;南昌市科技计划市校合作基金资助项目;No.20121228~~

摘　　要：目的:观察白介素-7(interleukin-7,IL-7)对小鼠肝纤维化模型肝组织病理的影响.方法:用CCl4诱导形成小鼠肝纤维化模型.将♀Balb/c小鼠18只随机分成3组,每组6只,A组(生理盐水对照组);B组(纤维化模型组);C组(IL-7干预组).造模4 wk末处死小鼠,取肝组织肉眼及组织病理形态学法(常规HE染色、网状纤维染色及Masson染色)观察肝组织标本,并采用Ishaki评分系统对小鼠肝组织炎症活动度及纤维化程度进行评分.结果:成功建立小鼠肝纤维化模型,表现为汇管区周围纤维化明显,有芒状纤维和纤维间隔形成,组织病理:HE染色、网状纤维染色及Masson染色显示均显示C组与B组相比,肝组织炎症及肝纤维化程度明显减轻,肝脏坏死性炎症分级Ishaki评分A组(0-3分)6只;B组(7-9分)1只,(10-14分)2只,(15-18分)3只;C组(4-6分)1只,(7-9分)3只,(10-14分)2只;3组炎症活动度得分有统计学差异(HC=14.14,P<0.01).肝纤维化分期Ishaki评分A组中0分5只,1分1只;B组4分2只,5分2只,6分2只;C组1分1只,2分1只,3分2只,4分2只;3组纤维化得分有统计学差异(HC=15.2,P<0.01).结论:IL-7能够阻断CCl4诱导的小鼠肝纤维化,减轻肝脏炎症反应.AIM： To observe the influence of interleukin-7 （IL-7） on hepatic pathology in mice with liver fibrosis. METHODS： Hepatic fibrosis was induced in mice by subcutaneous injection of CCI4. Eighteen female Balb/c mice were equally and randomly divided into three groups： a control group （A）,a fibrosis model group （B） and an IL-7 interven- tion group （C）. Four weeks after modeling, the mice were killed to observe liver tissues and pathological changes by HE staining, reticular fiber staining and Masson staining. The Ishaki scoring system was used to assess the severity of hepatic pathological changes. RESULTS： Hepatic fibrosis was successfully in- duced with CC14, and significant fibrosis around the portal area and formation of spiky fibers and fibrous septa were observed. Hematoxylin- eosin staining, reticular fiber staining and Mas- son staining showed that compared to group B, liver fibrosis and inflammation in group C were relieved significantly. There were no inflam- matory cells or fibers in group A. Based on the Ishaki scoring of the degree of inflammation, in group A 6 mice scored 0-3 points, 0 scored 4-6 points, 0 scored 7-9 points, 0 scored 10-14 points, and 0 scored 15-18 points; the corresponding figures were 0, 0, 1, 2, and 3 in group B and 0, 1, 3, 2, and 0 in group C. For hepatic fibrosis score, in group A 5, 1, 0, 0, 0, 0, and 0 mice scored 0 to 6 points, respectively, and the corresponding figures were 0, 0, 0, 0, 2, 2, and 2 in group B and 0, 1, 1, 2, 2, 0, and 0 in group C. There were signifi- cant differences in the degrees of inflammation and fibrosis between the three groups （P 〈 0.05）. CONCLUSION： IL-7 can mitigate hepatic fibro- sis and relieve liver inflammation in mice with hepatic fibrosis.

关 键 词：白介素-7 四氯化碳 肝纤维化 

分 类 号：R575.2[医药卫生—消化系统]