miR-22在肝细胞癌中的表达及其与上皮钙黏蛋白、波形蛋白的相关性  

miR-22 expression in hepatocellular carcinoma: Correlation with E-cadherin and Vimentin expression

作　　者：丁隆[1] 杨宇[2] 何淑玲[1] 夏伟滨[1] 曲义坤[1] 刘伟新[1] 徐剑[1] 张英海[1] 

机构地区：[1]佳木斯大学附属第一医院普外二科,黑龙江省佳木斯市154007 [2]佳木斯大学基础医学院局部解剖教研室,黑龙江省佳木斯市154007

出　　处：《世界华人消化杂志》2014年第21期3142-3147,共6页World Chinese Journal of Digestology

基　　金：黑龙江省卫生厅基金资助项目;No.2007-502~~

摘　　要：目的:探讨肝细胞癌(hepatocellular carcinoma,HCC)中microRNA-22(miR-22)表达及其在肿瘤细胞表型转化中的作用.方法:应用实时荧光定量PCR技术检测108例HCC及其对应癌旁组织和不同转移潜能的肝癌细胞MHCC97-L、Huh7中miR-22的表达情况;免疫组织化学SP法检测上述HCC及其对应癌旁组织中上皮钙黏蛋白(E-cadherin,E-cad)、波形蛋白(Vimentin)的表达;Pearson's软件分析miR-22的表达与E-cad、波形蛋白之间的相关性.结果:miR-22在HCC组织中的表达显著高于对应的癌旁组织(P<0.05).E-cad在HCC组织中的表达显著低于对应的癌旁组织(P<0.05),而HCC组织中波形蛋白表达显著高于对应的癌旁组织(P<0.05).Pearson's相关性分析表明,HCC组织中miR-22表达与E-cad呈负相关(r=-0.368,P<0.05),与波形蛋白表达呈正相关(r=1.475,P<0.05).间质表型的肝癌细胞MHCC97-L中miR-22表达显著高于上皮表型的肝癌细胞Huh7(P<0.05).结论:肝癌细胞高表达miR-22,且与E-cad表达呈负相关,而与波形蛋白表达呈正相关;miR-22可能是一个促进肝癌发生和转移的重要分子.AIM： To explore the expression of microRNA-22 （miR-22） in hepatocellular carcinoma （HCC） and its role in phenotype transition. METHODS： Real-time polymerase chain reac- tion （qPCR） was used to detect the expression of miR-22 in HCC tissues from 108 patients and in two liver cancer cell lines MHCC97-L and Huh7,which have different potential of metastasis. The expression of epithelial marker E-cadherin （E-cad） and mesenchymal marker Vimentin was evaluat- ed by the immunohistochemistry technique. The correlation between miR-22 expression and E-cad and Vimentin expression was analyzed. RESULTS： The expression of miR-22 was higher in HCC tissues than in matched tumor-adjacent tissues （P 〈 0.05）. The expression of E-cad was significantly lower and that of Vimentin was sig- nificantly higher in HCC tissues than in matched tumor-adjacent tissues （P 〈 0.05）. There was a negative correlation between miR-22 and E-cad expression （r = -0.368, P 〈 0.05）, but a positive correlation between miR-22 and Vimentin ex- pression （r = 1.475, P 〈 0.05）. Therefore, mes- enchymal MHCC97-L cells demonstrated high miR-22 expression, while epithelial Huh7 cells demonstrated low miR-22 expression, and the difference was significant between the two cell types （P 〈 0.05）. CONCLUSION： There is a negative correlation between miR-22 and E-cad expression and a positive correlation between miR-22 and Vimen- tin expression in HCC cells, miR-22 may be a vital factor contributing to the development and metastasis of HCC.

关 键 词：癌 肝细胞 miR-22 E钙黏蛋白 波形蛋白 

分 类 号：R735.7[医药卫生—肿瘤]