蔗糖与葡萄糖作辅助碳源对重组Klebsiella pneumoniae发酵生产1,3-丙二醇的影响  

Effect of Sucrose and Glucose as Cosubstrate on 1,3-Propanediol Fermentation by Recombinant Klebsiella pneumoniae

作　　者：吴家鑫[1,2] 王凤寰[1] 王峥[1] 谭天伟[1] 刘沛溢 

机构地区：[1]北京化工大学生命科学与技术学院北京市生物加工过程重点实验室,北京100029 [2]中牧实业股份有限公司 农业部兽用生物制品与化学药品重点实验室 北京市兽用多肽疫苗设计与制备工程技术研究中心,北京100095 [3]北京古龙嘉兰生物技术有限公司,北京100097

出　　处：《过程工程学报》2014年第4期660-663,共4页The Chinese Journal of Process Engineering

基　　金：国家重点基础研究发展计划(973)资助项目(编号:2007CB714304);国家高技术研究发展计划(863)资助项目(编号:2006AA020102;2006AA020103;2006AA020201)

摘　　要：实验研究了重组Klebsiella pneumoniae批式发酵生产1,3-丙二醇过程中辅助碳源蔗糖与葡萄糖对发酵过程的影响,对发酵工艺进行了放大,并对流加策略进行了优化.结果表明,葡萄糖为发酵生产1,3-丙二醇的辅助碳源优于蔗糖;以重组Klebsiella pneumoniae为菌种,以葡萄糖为辅助碳源,采用指数流加策略,30 L发酵罐中1,3-丙二醇的产量最高达85.2 g/L,产率达0.63 mol/mol,比单纯以甘油为碳源分别提高37.35%和25.00%.The effects of glucose and sucrose as two kinds of cosubstrate on fed-batch fermentation of 1,3-propanediol by recombinant Klebsiella pneumoniae were investigated, and the scale-up of fermentation technology and optimization of feeding strategy also studied. The results showed that glucose as cosubstrate was better than sucrose. The final yield and productivity of 1,3-propanediol were 85.2 g/L and 0.63 mol/mol in 30 L fermentor by exponential feeding strategy with glucose, respectively. Compared with only glycerol as the carbon source, the yield and productivity of 1,3- propanediol were raised by 37.35% and 25.00%, respectively.

关 键 词：1 3-丙二醇 重组Klebsiella PNEUMONIAE 辅助碳源 葡萄糖 发酵 指数流加 

分 类 号：Q815[生物学—生物工程]