miR-124参与紫杉醇诱导的乳腺癌细胞株MCF-7生长抑制  

The inhibitive effects of miR - 124 on the proliferation of breast cancer cell line MCF - 7 induced by pacfitaxel

作　　者：李文晶[1] 冯同保[1] 戚春建[1] 

机构地区：[1]南京医科大学附属常州市第二人民医院肿瘤研究所,常州213000

出　　处：《实用肿瘤学杂志》2014年第4期289-293,共5页Practical Oncology Journal

基　　金：国家自然科学基金项目（81272323）;常州市科技发展（社会发展项目CE20135044）;常州市卫生局重点项目（ZD201307）

摘　　要：目的探讨miR-124对紫杉醇诱导的乳腺癌细胞株MCF-7生长抑制作用的影响。方法应用MTr法检测紫杉醇对MCF-7的生长抑制作用，流式细胞仪检测紫杉醇对细胞周期的影响，应用实时荧光定量PCR（qRT-PCR）技术检测紫杉醇处理MCF-7细胞后，miR-124表达水平的变化，进一步转染miR-124抑制剂至MCF-7细胞中，用MTr法检测细胞生长抑制情况。结果MrI-T法检测结果显示紫杉醇能显著抑制MCF-7细胞生长。流式细胞仪检测结果显示紫杉醇能明显将细胞阻滞于G：期。qRT-PCR检测结果显示紫杉醇能诱导miR-124表达水平升高，并呈现剂量依赖关系。进一步研究发现，抑制细胞miR-124的表达后，紫杉醇对MCF-7的增殖抑制作用受到显著影响。结论紫杉醇能显著诱导MCF-7上调表达miR-124，并且这种上调表达是剂量依赖性的。而miR-124抑制剂能明显降低紫杉醇对MCF-7细胞的生长抑制作用。表明miR-124可能在细胞对紫杉醇的化疗耐药中起着重要作用，也为将来解决紫杉醇临床耐药提供了新思路。Objective Our study aims to investigate the inhibitive effects of miR - 124 on the growth of breast cancer cell MCF - 7 induced by paehtaxel. Methods MTr was used to detect the growth inhibition of MCF -7 induced by paclitaxel. Flow cytometry was used to detect the effect of paclitaxel on cell cycle. Real - time quantitative PCR（ qRT - PCR） was used to detect the expressive level of miR - 124, while MCF - 7 cells were treated with paclitaxel. MiR- 124 inhibitor was transfected into MCF-7 breast cancer cells,and growth in- hibition was detected by MTI＇. Results The results showed that paclitaxel could significantly inhibit the growth of breast cancer cell line MCF -7 by blocking the G2 phase. The results from qRT - PCR showed that the relative expression of miR - 124 was increased when the dosage of paclitaxel was increased. When the expression of miR - 124 was inhibited, the cell growth inhibition caused by paclitaxel was also prominently decreased. Conclusion The higher expression of miR - 124 in MCF - 7 induced by paclitaxel was dose dependent. And miR - 124 in- hibitor can significantly influence the cell growth inhibition caused by paclitaxel. These results indicat that miR - 124 plays an important role in paclitaxel - induced chemotherapy drug resistance, and provides a new direction to solve the problem.

关 键 词：miR-124 紫杉醇 乳腺癌 

分 类 号：R737.9[医药卫生—肿瘤]