ERCC1，TUBB3在上皮性卵巢癌中表达及其临床病理特征和相关性的研究  

Study on the association between expression and clinical and pathological significance of ERCC1 and TUBB3 in epithelial ovarian carcinoma

作　　者：陈可心[1] 李琛[1] 秦誉[1] 姚国栋[1] 耿敬姝[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院病理科,哈尔滨150081

出　　处：《实用肿瘤学杂志》2014年第4期294-298,共5页Practical Oncology Journal

基　　金：国家自然科学基金项目（81372785）;黑龙江省自然科学基金重点项目（41400453-8-13146）

摘　　要：目的研究核苷酸切除修复交叉互补组1（ERCC1）和TUBB3编码的β-tubulin-Ⅲ（3型B微管蛋白）在卵巢上皮性癌中的表达情况、病理特征及其临床意义，为个体化治疗提供一定的理论依据。方法本研究主要通过逆转录一实时荧光定量反应（RTq-PCR）方法检测上皮性卵巢癌组织中ER．CCI和TUBB3mRNA的表达情况，进一步分析上皮性卵巢癌临床病理特征与ERCC1，TUBB3的关系及二者之间的相关性。结果ERCC1和TUBB3在上皮性卵巢癌中均呈高表达，表达率分别为28．21％（22／78）和29．49％（23／78）。单因素分析ERCC1、TUBB3基因在上皮性卵巢癌高表达患者中仅与残余肿物大小有关（P〈0．05），二者与其他临床病理因素无关。ERCC1和TUBB3在上皮性卵巢癌中表达水平呈正相关（r=0．4249，P〈0．05）。结论上皮性卵巢癌组织存在不同水平的ERCC1和TUBB3基因mRNA，二者在癌组织中表达密切相关。ERCC1、TUBB3基因在上皮性卵巢癌中的表达与残余肿物大小呈正相关。Objective To investigate the expression of Excision repair cross complement goup 1 and β -Tubulin -Ⅲ in ovarian epithelial carcinoma and their correlation with pathological characteristics as well as clinical significance, thus to provide theoretical basis for ovarian epithelial carcinoma individualized treatment. Methods The mRNA expressions of ERCC1 and TUBB3 in ovarian epithelial cancer tissues were detected by reverse transcription real - time fluorescence quantitative reactin（ RTq - PCR）. We analyzed the correlation of the expression of ERCC1 mRNA and TUBB3 mRNA and clinical pathological features of patients. Results ERCC1 and TUBB3 positively expressed in epithelial ovarian cancer. The rate of high expression of ERCC1 was 28.21% （22/78） and that of TUBB3 high expression was 29.49% （23/78）. By univariate analysis, high expression of ER- CC1 mRNA and TUBB3 mRNA was only correlated with the size of residual mass（ P 〈 0.05）, but not other clinical and pathological features. ERCC1 and TUBB3 expression was moderately correlated with each other（ r = 0. 4249, P 〈 0.05 ）. Conclusion ERCC1 and TUBB3 positively expresses in epithelial ovarian cancer and they are correlated with each other. High expression of ERCC1 mRNA and TUBB3 mRNA is positively correlated with the size of resid- ual mass.

关 键 词：上皮性卵巢癌 核苷酸切除修复交叉互补组1 3型B微管蛋白 逆转录一实时荧光定量 反应 

分 类 号：R737.31[医药卫生—肿瘤] Q74[医药卫生—临床医学]