宫颈癌组织miR-221的表达及其与HPV感染的关联  

The expression of miR - 221 in cervical carcinoma and its relationship with HPV infection

作　　者：高冬梅[1] 张媛媛[1] 张璐[1] 朱明明[1] 马斌林[1] 

机构地区：[1]新疆医科大学附属肿瘤医院妇科,乌鲁木齐830011

出　　处：《实用肿瘤学杂志》2014年第4期299-304,共6页Practical Oncology Journal

基　　金：国家自然科学基金地区基金资助项目（81360302）

摘　　要：目的探讨miR-221在宫颈癌组织中的表达情况及其与HPV感染的关系。方法经HC2法检测宫颈癌患者高危型HPV病毒，分别收集30例高危型HPV（HR-HPV）阳性及5例阴性的宫颈癌标本。同时选取30例同期因良性疾病住院患者的正常宫颈组织为对照，通过RT-PCR方法检测宫颈组织中miR-221的表达情况，初步探讨miR-221与宫颈癌的发生发展及HPV感染的关系。并通过转染的方法分别将miR-221和anti-miR-221转入HPVl6阴性的宫颈癌细胞株C33a中和HPVl6阳性的宫颈癌细胞株Caski，观察其对C33a和Caski转移和侵袭的影响。结果miR-221在宫颈癌组织中的阳性表达率显著高于正常宫颈组织（P〈0．01），miR-221的表达水平与患者有无淋巴结转移、临床病理分级、临床分期密切相关（P〈0．01）；miR-221在HR—HPV阳性癌组织中的阳性表达率显著高于HR-HPV阴性癌组织（P〈0．01）；转染miR-221可促进Caski和C33a细胞转移和侵袭，而转入anti-miR-221可降低Caski和C33a细胞转移和侵袭能力，转染后两组细胞增殖率和凋亡率变化情况比较差异具有统计学意义（P〈0．05）。结论宫颈癌组织中miR-221表达升高与宫颈癌的发生发展及HPV感染密切相关。Objective The purpose of this study is to study the expression of miR -221 in cervical cancer tissues and its relationship with HPV infection. Methods HR - HPV infection was detected by HC2, and 30 cases of HR - HPV negative and 5 cases of HR - HPV positive cervical cancer tissues were collected. Mean- while,30 cases of normal cervical tissues in patients with benign disease were collected as control group. The ex- pression of miR- 221 was detected by RT- PCR ,preliminarily investigating the relationship between miR -221 expression and the occurrence of cervical cancer and HPV infection. Through transfeetion of miR -221 and anti - miR - 221 into HPVI6 - positive cervical carcinoma cell line Caski and HPVI6 - negative cervical carcinoma cell fine C33a, we observed the role of miR - 221 on the migration and invasion of Caski ceils and C33a ceils. Results Compared with normal cervical tissues, the expression of miR -221 in cervical cancer was significantly in- creased, the difference was statistically significant（ P 〈 0.01 ） ; and the expression of miR - 221 was closely correl- ative to the patients with or without lymph node metastasis, pathological grade and clinical stage （ P 〈 0.01 ） ; the expression of miR - 221 in HR - HPV positive cervical cancer tissues was higher than in HR - HPV negative cer- vical cancer tissues（P 〈 0.01 ） ; transfection of miR - 221 and anti - miR - 221 could promote or downregulate C33a and Caski cells migration and invasion, and the changes between two groups had statistical significance（ P 〈 0.05）. Conclusion The increased expression of miR - 221 in cervical cancer tissues is closely related to the oc- currence and development of cervical cancer and HPV infection.

关 键 词：宫颈癌 MIR-221 HPV感染 

分 类 号：R737.31[医药卫生—肿瘤] Q74[医药卫生—临床医学]