STAT3通过上调Twist基因表达从而诱导肺腺癌细胞上皮-间质转化  

STAT3 promotes lung adenocarcinoma EMT by up- regulating Twist expressing

作　　者：李志伟[1] 王凤云[1] 张倩倩[1] 李丁楠[1] 张纯慧[1] 燕飞虎[1] 张艳桥[1] 

机构地区：[1]哈尔滨医科大学附属肿瘤医院内科,哈尔滨150081

出　　处：《实用肿瘤学杂志》2014年第4期310-314,共5页Practical Oncology Journal

基　　金：黑龙江省教育厅科学技术研究面上项目（11541224）

摘　　要：目的探讨信号转导子与转录激活子3（STAT3）基因在肺腺癌细胞上皮-间质转化（Epi．thelial-mesenchymal transition，EMT）过程中的作用。方法应用STAT3-siRNA转染人肺腺癌A549细胞，下调STAT3基因表达。转染后观察细胞形态学变化，通过Westernblot检测STAT3和Twist蛋白的表达变化。同时应用Transwell法和划痕实验检测细胞的侵袭和迁移能力。并应用免疫组化法验证STAT3和Twist蛋白在肺腺癌组织中表达的相关性。结果STAT3-siRNA转染人肺腺癌A549细胞后，STAT3基因表达下降，同时降低Twist的表达，并且降低肺癌细胞的增殖、侵袭和迁移能力，在肺腺癌组织中我们也观察到随着分化程度升高，两个蛋白在肺癌组织中的的表达均逐渐降低，并呈正相关。结论STAT3基因通过调节Twist的表达，参与EMT过程，从而在肺癌进展中发挥重要作用。Objective To study effects of signal transducer and activator of transcription 3 （STAT3） gene on lung adenocarcinoma cells＇ EMT. Methods We observed morphological changes of the cells and detected the expression of STAT3 and Twist by western blot after STAT3 - siRNA plasmid was transfected into A549 cells. Meanwhile, we observed invasion and migration ability of A549 cells using transwell method and wound healing assay. Eventually, we verified the correlation between STAT3 and Twist expression in lung adenocarcinoma tissues by immunohistochemical method. Results si - STAT3 significantly down - regulated the expression of STAT3 in A549 cells, and pronouncedly inhibited migration and invasion of A549 cells in vitro. Meanwhile, we also found decreased expression of Twist. The expression of STAT3 was positively correlated with the expression of Twist in lung adenocarcinoma tissue specimens and the expression of two genes was reversely correlated with tissue differ- entiation degree. Conclusion The STAT3 participated in the process of EMT by regulating twist expression, which played a vital role in progression of lung adenocarcinoma.

关 键 词：肺腺癌 转导子与转录激活子3 TWIST 上皮-间质转化 

分 类 号：R734.2[医药卫生—肿瘤]