基于形态学与ITS序列对冠突散囊菌多样性研究  

Study on Polymorphisms of Eurotium cristatum by Morphology and ITS Sequence

作　　者：王文涛[1] 赵运林[2,3] 杨海君[3] 刘石泉[2,3] 胡治远[2] 

机构地区：[1]湖南农业大学植物保护学院,长沙410128 [2]湖南城市学院化学与环境工程学院,湖南益阳413000 [3]湖南农业大学生物科学与技术学院,长沙410128

出　　处：《中国农学通报》2014年第24期310-315,共6页Chinese Agricultural Science Bulletin

基　　金：湖南省自然科学基金项目"茯砖茶发酵优势菌多样性及微生态体系控制技术研究"(13JJ6074);湖南省高校产学研合作示范基地产业化项目"黑茶发酵工艺与质量控制关键技术"(13CY025);湖南省科学技术厅科技计划国际合作项目"茯砖茶降糖功能成分及其产品开发研究"(2012WK2013)

摘　　要：为研究茯砖茶中冠突散囊菌多样性的问题,以来自中国3个省份的5种茯砖茶为样品,从中分离出优势微生物金花菌4、16、B1、B3、B7,依托形态学与和分子生物学技术对菌株进行鉴定。结果发现,这5株菌株均属冠突散囊菌,并且存在着遗传多样性,其中16号菌株的电镜观察结果与其他4株菌株差异明显,且菌株子囊孢子的"赤道"周围存在较多小孔;在ITS序列构建系统发育树中4、B1、B3、B7聚类为一组,16号聚类成另一组,说明冠突散囊菌可能存在亚种,这与形态学结果一致。In order to study the diversity of Eurotium cristatum in brick tea, the author collected five samples ofbrick tea from three provinces in China, separated microorganisms golden flower bacteria of No.4, 16, B1, B3,B7, and identified bacterial strains based on the morphology and molecular biology techniques. Theexperimental results showed that the five strains were Eurotium cristatum and there was a genetic diversity ofdifferent sources of strains, the electron microscope results of No.16 strain had the greatest difference fromothers, which had little holes around＂equator＂of ascospore. In ITS sequence of phylogenetic tree, cluster ofNo.4, B1, B3 and B7 was one set, cluster of No. 6 was another set. There may be a subspecies in Eurotiumcristatum, which consistent with the morphological results.

关 键 词：冠突散囊菌 ITS 形态学 多样性 拮抗 

分 类 号：Q939.5[生物学—微生物学]