热激启动子18.2在金鱼草中启动下游基因表达最适温度条件的筛选  

Optimal temperature for hsp18.2 promoter in gene expression of anthocyanin biosynthesis of Antirrhinum majus

作　　者：宋倩娜 刘琛[1] 高振蕊[1,2] 李莹[1,2] 张弛[1,2] 崔敏龙 

机构地区：[1]中国科学院沈阳应用生态研究所,污染生态与环境工程重点实验室,沈阳110016 [2]中国科学院大学,北京100049

出　　处：《生态学杂志》2014年第9期2436-2441,共6页Chinese Journal of Ecology

基　　金：云南省科技计划项目(2012IB011);国家自然科学基金项目(31370526)资助

摘　　要：为了研究温度对植物根系中基因表达的影响,构建了由拟南芥小热激蛋白18.2基因的启动子和金鱼草中调控花青素生物合成的基因(Rosea1)组成的植物表达载体pBIPhsp::ROS,利用遗传转化法得到毛状根,以根系颜色变化为指标,探讨了温度变化对植物根系基因表达的影响。结果表明:37、40和42℃热激处理4 h后,在毛状根中均能观察到一定程度的花青素积累;在42℃条件下处理8 h时,转基因毛状根颜色变化最深;RT-PCR分析表明,Rosea1基因的表达水平与毛状根着色呈正相关。该体系可作为温度对植物根系影响研究的一种指示标记和手段。To clarify the impact of temperature change on gene expression in roots, we made a plant expression vector pBIPhsp： ：ROS, which harbors a MYB gene Roseal controlling anthocyanin biosynthesis in Antirrhinum majus and the promoter of small heat-shock protein 18.2 of Arabidopsis. We obtained hairy roots by genetic transformation. Moreover, we investigated the impact of temperature change on gene expression in roots. The results showed that anthocyanin accumulations were observed in the transgenic hairy roots under treatment of 37, 40 and 42 ℃ for 4 h, and the deepest coloration was detected in the treatment of 42 ℃ for 8 h. In addition, by the method of RT-PCR, the expression of Roseal was detected in the hairy roots treated at 37, 40 and 42 ℃, and a significant correlation between expression pattern and anthocyanin accumulation was found. Therefore, our result suggests for the study of the effect of temperature c that the pBIPhsp： ：ROS is a useful marker and a tool hange on gene expression in roots.

关 键 词：转基因毛状根 热激处理 颜色 RT-PCR分析 

分 类 号：Q943.2[生物学—植物学]