液质联用法测定化妆品中3种蒽醌类化合物的含量  

Determination of Three Anthraquinone Compounds in Cosmetics by Liquid Chromatography Tandem Mass Spectrometry

作　　者：虞成华[1] 杜茹芸 黎超[1] 聂磊[1] 王承平[1] 

机构地区：[1]上海市质量监督检验技术研究院,上海200233

出　　处：《香料香精化妆品》2014年第4期42-44,48,共4页Flavour Fragrance Cosmetics

基　　金：质检公益性行业科研专项(编号2012104013-2)

摘　　要：建立了液相色谱-串联质谱联用测定化妆品中3种芦荟蒽醌类化合物含量的方法。样品经甲醇超声提取,用高效液相色谱分离后,采用电喷雾串联质谱进行定性和定量检测。方法线性范围为10-100 ng/mL,相关系数r大于0.99,最低检出限为3 ng/mL（S/N=3）,高、中、低3个浓度水平的加标回收率在80.4%-95.2%之间,测定值的相对标准偏差（n=6）小于10%。A sensitive method for determination of three anthraquinone compounds in cosmetics by liquid chromatography tan dem mass spectrometry（LC-MS/MS） was established. The cosmetics sample was ultrasonically extracted by methanol, then was separated and delermined by HPLC-ESI-MS/MS. A linear calibration curve was obtained in the concentration range of 10 to 100 ng/ml.. The linear correlation coefficients of the method were above 0.99. The detection limit of method was 3 ng/mL （S/N = 3）. The recoveries at three levels ranged from 80.4% to 95.2%, with the relative standard deviation （n = 6） of less than 10%

关 键 词：高效液相色谱法 串联质谱法 化妆品 芦荟苷 芦荟大黄素 大黄酚 

分 类 号：O657.63[理学—分析化学] TQ658[理学—化学]