Differential Proteomics Analysis on Fluoride-resistant Streptococcus mutans by Label-free Quantitation  

作　　者：ZHAO Hongyan ZHANG Zhimin ZHU Laikuan LI Zhenling LI Penglian LIU Jiabai ZHANG Zhiying 

机构地区：[1]School of Stomatology, Jilin University, Changchun 130021, P. R. China [2]Shanghai Key Laboratory of Stomatology, The Ninth People's Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai 200011, P. R. China

出　　处：《Chemical Research in Chinese Universities》2014年第4期566-571,共6页高等学校化学研究（英文版）

基　　金：Supported by the National Natural Science Foundation of China（Nos.81170945, 81200774）.

摘　　要：To figure out the changed biological characteristics of a fluoride-resistant strain compared with those of the parent sensitive strain of Streptococcus mutans（S,mutans） with the help of differential proteomics analysis,sixtyseven proteins with obvious differential expression were identified in fluoride-resistant strain.Among them are the important virulence factors causing caries,including glucosyltransferase-S,glucosyltransferase-I,glucosyltransferase-Si,major cell-surface adhesin Pac,F-ATPase β-subunit,Fth,molecular chaperone DnaK,chaperonin GroEL,and the lactate dehydrogenase.We obtained the differential proteomics expression profiles of the fluoride-resistant strain（UA159-FR） and its parental strain（UA159） of S.mutans.Several important virulence factors bringing about caries were identified to have differential expressions,among which the expression of lactate dehydrogenase in fluoride-resistant strain was enhanced compared to that in its parent strain.The results could provide the basis for analvsing the functions of proteins encoded by caries-related genes from the fluoride-resistant S.mutans strain.To figure out the changed biological characteristics of a fluoride-resistant strain compared with those of the parent sensitive strain of Streptococcus mutans（S,mutans） with the help of differential proteomics analysis,sixtyseven proteins with obvious differential expression were identified in fluoride-resistant strain.Among them are the important virulence factors causing caries,including glucosyltransferase-S,glucosyltransferase-I,glucosyltransferase-Si,major cell-surface adhesin Pac,F-ATPase β-subunit,Fth,molecular chaperone DnaK,chaperonin GroEL,and the lactate dehydrogenase.We obtained the differential proteomics expression profiles of the fluoride-resistant strain（UA159-FR） and its parental strain（UA159） of S.mutans.Several important virulence factors bringing about caries were identified to have differential expressions,among which the expression of lactate dehydrogenase in fluoride-resistant strain was enhanced compared to that in its parent strain.The results could provide the basis for analvsing the functions of proteins encoded by caries-related genes from the fluoride-resistant S.mutans strain.

关 键 词：Fluoride-resistant Streptococcus mutans Differential proteomics Label-free quantitation 

分 类 号：Q78[生物学—分子生物学] TS272.7[农业科学—茶叶生产加工]