气相色谱-串联质谱联用法测定水中丁香酚残留  

Determination of eugenol in water using gas chromatography coupled to tandem mass spectrometry

作　　者：柯常亮[1,2] 刘奇[1,2] 陈洁文 甘居利[1,2] 陈培基 李刘冬[1,2] 

机构地区：[1]中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室 [2]农业部水产品贮藏与质量安全风险评估实验室(广州),广东广州510300

出　　处：《中国渔业质量与标准》2014年第4期49-55,共7页Chinese Fishery Quality and Standards

基　　金：中央级公益性科研院所基本科研业务费专项(2010TS08和2012TS15)

摘　　要：为排查流通环节鱼用麻醉剂的潜在风险,本研究建立了水中丁香酚残留的测定方法.本方法以乙酸乙酯作为提取剂,使用气相色谱-串联质谱仪对丁香酚进行定性和定量测定.结果显示,在1.00～200.00 μg/L的浓度范围内,标准曲线方程为y=14.65 x-7.23,相关系数r为0.999 4,线性拟合良好.加标浓度在5.00～100.00 μg/L范围内回收率范围为98.6％ ～ 104％,相对标准偏差范围为2.46％ ～ 5.48％.方法检测限和定量限分别为0.21和0.68 μg/L.本方法准确、简单、快捷,能够满足鱼类产品流通环节水中丁香酚残留的测定.In order to investigate the potential risk of anesthetic application in fish transportation, the method was developed to determine residue of eugenol in water. The target compound in water sample was extracted using ethyl acetate, and then conducting the qualitative and quantitative determination by using gas chromatography coupled to tandem mass spectrometry （ GC - MS/MS）. As shown, within 1.00 - 200.00 μg / L concentration range of the standard curve equation y = 14.65 × - 7.23, correlation coefficient （r） is 0. 999 4, the linear fit is good. While spiked concentration from 5.00 to 100.00 μg/L, the recoveries of eugenol ranged from 98.6% to 104%, and the relative stand ard deviation （RSD） were ranged from 2.46% to 5.48%. The limit of detection （LOD） and limit of quantification （LOQ） values were 0.21 and 0.68μg/L, respectively. It can be concluded that the method is accurate, simple, fast and qualified to detect eugenol residue in water during fish transporting.

关 键 词：丁香酚 气相色谱-串联质谱 水 麻醉剂 

分 类 号：S948[农业科学—水产养殖]