脾肾合剂对顺铂诱导的SD大鼠胃肠损伤的影响  

Effect of Spleen and Kidney Mixture on Cisplatin-induced Gastrointestinal Injury in SD Rats

作　　者：姚德蛟[1] 陈玉[1] 蔡懿[1] 杨俊[1] 丁芋友[2] 刘俊[2] 孟春芹[2] 

机构地区：[1]成都中医药大学附属医院肿瘤科,成都610072 [2]成都中医药大学临床医学院,成都610075

出　　处：《中西医结合研究》2014年第4期184-187,共4页Research of Integrated Traditional Chinese and Western Medicine

基　　金：四川省中医药管理局资助课题(No.2012-F-021)

摘　　要：目的探讨脾肾合剂对顺铂诱导的SD大鼠胃肠损伤的影响。方法运用尾静脉注射顺铂建立SD大鼠胃肠损伤的模型,动物随机分为对照组、模型组、格拉司琼组、脾肾合剂组,给予相应药物干预,观察大鼠进食量和体重情况,采用酶联免疫法(ELISA)检测血清表皮生长因子(EGF)水平,采用免疫组化法检测十二指肠嗜铬细胞5-羟色胺(5-HT)的表达。结果与模型组比较,脾肾合剂组大鼠进食量增加,体重增加,血清EGF水平升高(P<0.01),十二指肠嗜铬细胞5-HT表达下降(P<0.05)。结论脾肾合剂可改善顺铂诱导的SD大鼠胃肠损伤。Objective To explore the effect of spleen and kidney mixture on cisplatin-induced gastrointestinal injury in SD rats.Methods T he rat model of cisplatin-induced gastrointestinal injury w as established by injecting cisplatin into tail vein of SD rats.Animals were randomly divided into control group ,model group ,granisetron group ,spleen and kidney mixture group.The rats in different groups received the corresponding therapy.The daily food intake and body weight of rats were measured.Serum level of epidermal growth factor（EGF）was examined by enzyme-linked immunoassay（ELISA）.The protein expression of 5-HT in chromaffin cells of duodenum was evalua-ted immunohistochemical method.Results Compared with model group ,daily food intake ,body weight and serum EGF levels were significantly increased in rats of spleen and kidney mixture group P〈0.01）.However ,the protein expression of 5-HT in chromaffin cells of duodenum was decreased in rats of spleen and kidney mixture group P〈0.05）.Conclusion The spleen and kidney mixture could improve cisplatin-induced gastrointestinal tract injury in SD rats.

关 键 词：脾肾合剂 顺铂 胃肠道损伤 5-羟色胺 

分 类 号：R73-36[医药卫生—肿瘤]