血糖水平对缺血再灌注脑组织过氧化损伤的影响  

Effect of blood glucose level on cerebral ischemia-reperfusion injury of rats

作　　者：任歆 黄蕊 成学恭 李光来 

机构地区：[1]煤炭总医院神经内科,北京100028

出　　处：《中国医药导报》2014年第24期36-40,共5页China Medical Herald

摘　　要：目的：探讨在缺血再灌注脑损伤动物模型中血糖水平对氧化应激反应的影响。方法采用结扎大鼠两侧颈总动脉的急性脑缺血再灌注模型，Wistar雄性大鼠随机分为4组：假手术组（A组）、生理盐水对照组（B组）、胰岛素组（2.1 U/kg，C组）、胰岛素（2.1 U/kg）＋50%葡萄糖（2 g/kg）组（D组）。术后取标本切片观察脑组织超微结构的变化，并检测脑组织中丙二醛（MDA，脂质过氧化产物）、超氧化物歧化酶（SOD）含量及ATP酶活性。结果①A、D组血糖正常（4.6-10 mmol/L），与之相比，B组血糖升高明显（P〈0.01），C组血糖下降明显（P〈0.01）；②脑组织内MDA、SOD含量及ATP酶活性，B组与C组相比差异无统计学意义（P〉0.05），同B组相比，D组血糖水平在正常值的高限可以降低脑组织中MDA含量（P〈0.05），升高SOD水平（P〈0.05），提高ATP酶活性（P〈0.05）。结论①脑缺血再灌注损伤可以导致血糖水平升高并产生氧化应激损伤；②缺血再灌注时低血糖也可以加剧氧化应激反应；③缺血再灌注后控制血糖至正常值的高限可以减轻氧化应激反应。Objective To discuss the influence of blood glucose on oxidative stress in animal cerebral ischemic-reper-fusion models. Methods Acute cerebral ischemia-reperfusion models were made by bilateral carotid artery ligation for 30 minutes and then reperfusion for 2 hours. Male Wistar rats were randomly divided into 4 groups： sham operation group （group A）, normal saline control group （group B）, insulin group （2.1 U/kg, group C）, insulin （2.1 U/kg） and 50%glucose （2 g/kg） group （group D）. After operation, the brain tissue specimens were sectioned to observe the change of ultrastructure. The content of methane dicarboxylic aldehyde （MDA, product of lipid peroxidation）, superoxide dismu-tase （SOD）, and the activity of ATP enzyme were detected. Results ①Blood glucose levels in group A and D were within normal limits （4.6-10 mmol/L）, blood glucose in group B increased significantly （P〈 0.01）, blood glucose in group C decreased significantly （P〈0.01）;②MDA and SOD content, ATP-ase activity in brain tissue of group B and group C had no significant difference （P〉0.05）;compared with the group B, MDA was reduced （P〈0.05）, SOD was elevated （P〈 0.05）, and ATP-ase activity was increased （P〈 0.05） in group D. Conclusion ①Cerebral ischemia-reperfusion can increase blood glucose, and cause oxidative stress in cerebral tissue. ②During ischemia reperfusion, if blood glucose level is too low, can aggravate oxidative stress. ③Control blood glucose to upper limit of normal range can reduce oxidative stress.

关 键 词：血糖 脑缺血-再灌注 氧化应激 

分 类 号：R743.3[医药卫生—神经病学与精神病学]