HIV^+牙周炎患者龈沟液IL-6的检测分析  

Analysis of IL-6 in gingival crevicular fluid of HIV-positive patients with periodontitis

作　　者：侯雯[1] 贾洪诚[1] 王璇[2] 华文浩[1] 付茜[1] 李晓光[1] 

机构地区：[1]首都医科大学附属北京地坛医院口腔科,100015 [2]首都医科大学附属北京同仁医院口腔科

出　　处：《中华临床医师杂志（电子版）》2014年第15期23-26,共4页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的初步探讨HIV+牙周炎患者龈沟液中IL-6和CD4+T淋巴细胞计数与牙周临床指标的相关性,为临床提供参考。方法记录20例HIV+牙周炎患者共120颗指数牙的牙周临床指标:菌斑指数(PLI)、出血指数(BI)、附着水平(AL)和牙周探诊深度(PD),用放射免疫法检测龈沟液中IL-6。计数受试者血液的CD4+T淋巴细胞,并按其分组:A组(CD4计数>500 cells/mm3,2例12颗牙),B组(CD4计数200~500 cells/mm3,13例78颗牙),C组(CD4计数<200 cells/mm3,5例30颗牙)。组间牙周临床指标和IL-6比较采用Mann-Whitney秩和检验,CD4+T淋巴细胞计数和牙周临床指标的关系采用偏相关分析,IL-6和牙周临床指标的关系采用Spearman相关分析。结果 B组的BI值、GCF值、IL-6浓度分别为3.00(2.00)、4.80(2.20)ml、10.36(5.54)pg/μl。三组间的PD、AL差异均无统计学意义。B组和C组的CD4计数和BI有相关性(P<0.05)。IL-6含量与各项牙周临床指标呈正相关(P<0.05);IL-6浓度与各项牙周临床指标呈负相关(P<0.05)。结论 HIV+牙周炎患者的牙周炎症程度与CD4+T淋巴细胞计数有关,其龈沟液中IL-6水平与牙周临床指标有关。Objective To investigate the relationship between IL-6, CD4＋T lymphocyte counts and periodontal status of HIV＋patients with periodontitis. Methods The gingival crevicular fluid were collected from 120 teeth of 20 patients. The levels of IL-6 were determined by RIA assays. Clinical measurements were recorded, including plaque index（PLI）, bleeding index（BI）, attachment level（AL） and probing depth（PD）. The plasmatic CD4＋T lymphocytes were counted. All the individuals were divided into three groups：A group（CD4＆gt;500 cells/mm3）, B group（200 cells/mm3≤CD4≤500 cells/mm3） and C group （CD4〈200 cells/mm3）. Mann-Whitney were used to compare groups. Partial correlations and Spearman correlations were applied to analyze the correlation of CD4 cell counts and IL-6 with periodontal status. Results BI, GCF, and IL-6 concentration were 3. 00（2.00）, 4. 80（2.20）ml, and 10. 36（5.54）pg/μl, in the B group. The differences of them were statistically significant between group B and A（P〈0.05）. But the differences of PD and AL among three groups were of no statistical significance. The correlations were observed between CD4 cell counts and BI with group B and C（P〈0.05）. IL-6 concentration was negatively correlated with BI, PD, GCF and AL（P〈0.05）, while total IL-6 was positively correlated with them（P〈0.05）. Conclusion The findings suggested that IL-6 might be associated with periodontal status of HIV＋patients with periodontitis. There was complex association between periodontal status and CD4 cell counts in HIV＋patients.

关 键 词：CD4阳性T淋巴细胞 白细胞介素6 HIV 牙周炎 

分 类 号：R781.4[医药卫生—口腔医学]