细胞代谢组学用于木犀草素抑制MCF-7细胞的机制研究  

Metabonomics Study on Luteolin Intervention of Breast Cancer MCF-7 Cells

作　　者：史栋栋 王桂明[1] 况媛媛[1] 彭章晓[1] 王彦[1] 谷雪[1] 阎超[1] 

机构地区：[1]上海交通大学药学院,上海200240

出　　处：《分析化学》2014年第8期1088-1093,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.21175092;21105064);国家重大科学仪器设备开发专项(Nos.2011YQ150072;2011YQ15007204;2011YQ15007207;2011YQ15007210);上海市自然科学基金项目(No.12ZR1413600)基金资助项目~~

摘　　要：基于气相色谱质谱联用(GC-MS)技术将代谢组学的方法结合细胞周期的实验,研究木犀草素作用于MCF-7细胞的作用机理。细胞活性实验验证,木犀草素对MCF-7细胞有抑制作用,GC-TOF/MS对加药细胞和未加药细胞代谢物进行指纹图谱分析,并进一步应用偏最小二乘判别分析(OPLS-DA)对代谢组学数据进行多维统计分析。结合木犀草素将细胞周期抑制在S期(Synthesis),推测木犀草素通过阻碍核酸代谢中的磷酸戊糖途径抑制MCF-7细胞的增殖。The metabolic profiles of control and MCF-7 cells treated with luteolin were analyzed separately using gas chromatography/mass spectrometry （GC/MS） to study the mechanism of the luteolin treatment on MCF-7 cells. Cell viability assays showed that luteolin had inhibition effect on MCF-7 cells. Partial least square discriminant analysis （OPLS-DA） was used to process the metabolic data. Since ceils in phase of S were increased significantly, we speculated that luteolin had a blocking effect on pentose phosphate pathway of MCF-7 cells, which contributed to its inhibition effect on proliferation of MCF-7 cells.

关 键 词：气相色谱质谱联用 细胞代谢组学 MCF-7细胞 细胞周期 乳腺癌 木犀草素 

分 类 号：R285[医药卫生—中药学] O657.71[医药卫生—中医学]