缺血后处理对大鼠肾缺血再灌注时c-fos蛋白表达的影响  

Effects of ischemic postconditioning on c-fos protein expression during renal ischemia-reperfusion in rats

作　　者：田明[1] 马宏仲[1] 姜秀良[1] 梁立升[1] 李爱芝[1] 马加海[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院麻醉科,264000

出　　处：《中华麻醉学杂志》2014年第6期758-760,共3页Chinese Journal of Anesthesiology

基　　金：烟台市科技发展计划项目（2009155-12）

摘　　要：目的评价缺血后处理对大鼠肾缺血再灌注时c-fos蛋白表达的影响。方法成年雄性sD大鼠75只，8～12周龄，体重200～250g，采用随机数字表法，将其分为3组（n=25）：假手术组（S组）、缺血再灌注组（I／R组）和缺血后处理组（IPO组）。I／R组和IPO组采用结扎右侧肾蒂，夹闭左侧肾蒂1h，再灌注24h的方法制备大鼠肾缺血再灌注损伤模型。分别于再灌注1、3、6、12和24h时，处死5只大鼠，分离左肾组织，光镜下观察病理学结果，采用免疫组化法检测c-fos蛋白表达。结果与S组比较，I／R组和IPO组再灌注各时点肾组织c-fos蛋白表达上调（P〈0．05），病理学损伤明显；与I／R组比较，IPO组再灌注各时点肾组织c-fos蛋白表达下调（P〈0．05），病理学损伤减轻。结论缺血后处理减轻大鼠肾缺血再灌注损伤的机制与下调肾组织c-fos蛋白表达有关。Objective TO evaluate the effects of isehemic postconditioning （IPO） on c-fos protein expression during renal ischemia-reperfusion （I/R） in rats. Methods Seventy-five adult male Spragne-Dawley rats, aged 8-12 weeks, weighing 200-250 g, were randomly allocated into 3 groups （ n = 25 each） using a random number table： sham operation group （group S）, I/R group and IPO group. Renal I/R injury was induced by damping the bilateral renal pedicles for 1 h followed by 24 h of reperfusion in I/R and IPO groups. Five animals were sacrificed at 1, 3, 6, 12 and 24 h of reperfusion and the left kidneys were removed for microscopic examination and for determination of the expression of c-fos protein by immunohistochemistry. Results Compared with S group, the expression of c-fos protein was significantly up-regulated at each time point during reperfusion in I/R and IPO groups. Compared with I/R group, the expression of c-fos protein was significantly down-regulated at each time point during reperfusiou in group IPO. The pathologic changes were significantly attenuated in group IPO as compared with group I/R. Conclusion The mechanism by which IPO attenuates renal I/R injury is related to down-regnlatiou of c-fos protein expression in the renal tissues of rats.

关 键 词：再灌注损伤 肾 原癌基因蛋白质C-FOS 缺血后处理 

分 类 号：R363[医药卫生—病理学]