高效液相色谱法同时测定新型香料毒品中的10种合成大麻素  

Simultaneous Determination of 10 Synthetic Cannabinoids in Novel“Spice”Drugs by High Performance Liquid Chromatography

作　　者：翟晚枫[1] 张春水[1] 高利生[1] 

机构地区：[1]公安部物证鉴定中心,北京100038

出　　处：《分析测试学报》2014年第8期893-898,共6页Journal of Instrumental Analysis

基　　金：公安部重点研究计划项目(201201ZDYJ005)

摘　　要：建立了高效液相色谱法同时测定新型香料毒品中10种合成大麻素的分析方法.样品以甲醇提取,离心、过滤后,采用Shim-pack XR-ODS C18（4.6 mm×250 mm,5μm）色谱柱分离,以甲醇-乙腈（50＞50）和水作为流动相进行梯度洗脱,柱温45℃,流速1.0 mL/min,检测波长220 nm.结果表明,该方法可在33 min内实现对常见10种合成大麻素的完全分离和定量,在1～100 mg/L线性范围内,相关系数均为0.999 9,检出限为0.10～0.50 mg/L.加标回收率为98.2％～102.1％,日内相对标准偏差（RSD）为0.15％～1.37％,日间相对标准偏差为0.55％～1.96％.样本在室温放置96 h、-20℃放置15 d以及在室温和-20℃反复冻融3次条件下稳定性均良好.该方法准确、快速、灵敏、分离效果好,适用于新型香料毒品中常见合成大麻素成分的检测.A high performance liquid chromatographic method was developed for the simultaneous determination of ten synthetic cannabinoids in novel ＂spice＂ drugs.A Shim-pack XR-ODS C18（4.6 mm× 250 mm,5 μm） column was used at 45 ℃.The mobile phase system contained methanol-acetonitrile（50 ∶ 50） and water,and the percentage of organic phase changed between 66％and 89.1％ in a linear gradient elution.The flow rate was set at 1.0 mL/min,and the detection wavelength was 220 nm.Under the optimized conditions,ten synthetic cannabinoids were completely separated and determined in 33 min.Good linear relationships were obtained in the range of 1-100mg/L with correlation coefficients of 0.999 9.The LODs were in the range of 0.10-0.50 mg/L.The intra-day relative standard deviations（RSDs） were in the range of 0.15％-1.37％ and the interday RSDs were 0.55％-1.96％.The sample showed good stability under room temperature,freeze and three cycles of freeze-thaw conditions.This method was accurate,sensitive and rapid for the determination of common synthetic cannabinoids in novel ＂spice＂ drugs.

关 键 词：高效液相色谱 香料 合成大麻素 

分 类 号：O657.72[理学—分析化学] S859.81[理学—化学]