TGF-β1及Ⅰ型受体ALK1在人脑胶质瘤细胞的表达及意义  

Expression and significance of TGF-β1 and its type Ⅰ receptor ALK1 in human brain glioma

作　　者：冯九庚[1] 封荣华[2] 洪涛[1] 徐姗[3] 杨玄勇[1] 曾春辉[1] 

机构地区：[1]南昌大学第一附属医院神经外科,南昌330006 [2]江西省中医院功能诊断科,南昌330006 [3]南昌大学第一附属医院病理科,南昌330006

出　　处：《重庆医学》2014年第24期3139-3142,共4页Chongqing medicine

基　　金：江西省卫生厅科技计划项目(20111037);国家自然科学基金资助项目(81060099)

摘　　要：目的观察转化生长因子β1(TGF-β1)及活化素受体样激酶1(ALK1)在人脑胶质瘤细胞中的表达及其在胶质细胞瘤病理分级中的意义。方法采用半定量RT-PCR、Western blot及免疫组织化学染色等方法检测32例人脑胶质瘤及3例正常脑组织中TGF-β1及ALK1mRNA及蛋白的表达水平。结果人脑胶质瘤细胞中存在TGF-β1及ALK1mRNA和蛋白表达。与正常组比较,高级别胶质瘤组TGF-β1及ALK1mRNA和蛋白表达均显著升高(P<0.05),其中ALK1的高表达与胶质瘤病理级别呈明显正相关(r=0.297,P<0.05)。结论人脑胶质细胞瘤中,ALK1可能参与了胶质瘤的恶变过程。Objective To study the expression of transforming growth factor beta1（TGF-β1）and its type I receptors activin-like kinase 1（ALK1）in the human brain glioma and estimate the significance in the pathogenisis and development of human glioma . Methods The mRNA and protein expressions of TGF-β1 and ALK1 were detected with semiquantitative RT-PCR ,Western blot and Immunohistochemistry in 3 normal brain tissues and 32 patiens with human glioma .Results ALK1 and TGF-β1 mRNA and protein sometimes had co-expression in human gliomas tissues .Compared with those normal brain tissues ,the expression of TGF-β1 and ALK1 mRNA and protein in high grade gliomas were significantly increased （P〈0 .05） ,the overexpression of ALK1 in human gliomas was demonstrated significant positive correlation with the pathological grades of gliomas （r=0 .297 ,P〈0 .05） .Conclusion The overexpression of ALK1 may play important role in the development of human brain glioma .

关 键 词：转化生长因子Β1 活化素受体样激酶1 脑胶质瘤 

分 类 号：R739.4[医药卫生—肿瘤]