木瓜籽精油的提取及其抗氧化活性研究  

Extraction and Antioxidant Activity of Essential Oil from the Papaya Seeds

作　　者：邓福明[1,2,3] 梁淑云[1,2,3] 陈卫军[1,2,3] 王挥[1,2,3] 赵松林[1,2,3] 

机构地区：[1]中国热带农业科学院椰子研究所,海南文昌571339 [2]国家重要热带作物工程技术研究中心,海南文昌571339 [3]海南省椰子深加工工程技术研究中心,海南文昌571339

出　　处：《热带农业科学》2014年第7期88-92,共5页Chinese Journal of Tropical Agriculture

基　　金：国家重大科技成果转化项目"重要热带作物产品加工关键技术产业化应用";中国热带农业科学院科技开发启动资金项目(No.2013-kfqdj-03)

摘　　要：采用水蒸气蒸馏法提取木瓜籽精油(PSO),测定PSO的总抗氧化能力、羟基自由基和DPPH自由基清除能力,并应用GC-MS分析测定PSO的化学组成。结果表明,水蒸气蒸馏法提取木瓜籽精油最佳蒸馏时间是2.5 h,料液比和浸泡时间分别是1∶8(w∶V)和1 h;与合成抗氧化剂相比,PSO的总抗氧化能力偏低,当PSO浓度达到5×10-5mg/mL和8 mg/mL时,其清除羟基自由基和DPPH自由基的能力分别达到100%和70%以上;PSO化学成分主要是安息香醛、苯乙醛、苯乙腈和异硫氰酸苄酯,其中异硫氰酸苄酯的相对含量占98.28%。说明该方法可获得高纯度的异硫氰酸苄酯,可为工业上生产异硫氰酸苄酯提供一定的理论依据。The papaya seed oil（PSO） was extracted by steam distillation in this paper. Then the total antioxidant capacity, hydroxyl free radicals and DPPH free radicals scavenging of the PSO were determined. Finally, the chemical composition was analyzed using GC-MS. Results indicate that the Optimal distillation time and solid-liquid ratio and soaking time is 2.5 h, 1/8 and 1 h, respectively.Compare to the synthetic antioxidant, the total antioxidant capacity of the PSO was lower. However, the hydroxyl free radicals and DPPH free radicals scavenging of the PSO reach to 100% and 70% above while the concentration of the PSO was 5×10^-5mg/mL and 8 mg/mL, respectively. The chemical composition of the PSO is mainly Benzaldehyde, Benzeneacetaldehyde, Benzyl nitrile and Isothiocyanic acid-benzyl ester,98.28% of which is Benzyl nitrile and Isothiocyanic acid. This results show that the method can obtain high purity isothiocyanate benzyl ester, in order to provide a theoretical basis for the industrial production isothiocyanate benzyl ester.

关 键 词：木瓜籽 精油 提取 抗氧化活性 

分 类 号：S661.6[农业科学—果树学]