随机扩增多态性DNA技术研究发现二氧化钛纳米颗粒对西葫芦具有基因毒性(英文)  

Random amplified polymorphic DNA reveals that TiO_2 nanoparticles are genotoxic to Cucurbita pepo

作　　者：Fabiola MORENO-OLIVAS Vincent U.GANT Jr. Kyle L.JOHNSON Jose R.PERALTA-VIDEA Jorge L.GARDEA-TORRESDEY 

机构地区：[1]Depertment of Chemistry, The University of Texas at El Paso, El Paso, TX 79968, USA [2]University of California Center for Environmental Implications of Nanotechnology (UC CEIN) [3]The University of Texas at El Paso, El Paso, TX 79968, USA [4]Department of Biological Sciences and Border Biomedical Research Center, The University of Texas at El Paso, El Paso, TX 79968, USA [5]Environmental Science and Engineering PhD Program, The University of Texas at El Paso, El Paso, TX 79968, USA

出　　处：《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》2014年第8期618-623,共6页浙江大学学报（英文版）A辑（应用物理与工程）

基　　金：Project supported by the National Science Foundation(NSF);the Environmental Protection Agency under Cooperative Agreement(No.DBI-0830117);the National Institutes of Health(NIH)Grant(No.2R25GM069621-09);the National Institute on Minority Health and Health Disparities(NIMHD)Grant(No.8G12MD007592);the United States Department of Agriculture(USDA)Grant(No.2011-38422-30835);the Research and Engineering Apprenticeship Program(REAP)Grant(No.W11NF-10-2-0076),USA

摘　　要：研究目的:二氧化钛(TiO2)纳米颗粒已经广泛应用于化妆品、防晒霜、涂料和牙膏等。这些纳米颗粒性质非常稳定,能在废水和生物固体中转移和分散。现有研究表明,TiO2纳米颗粒对动物正常生理活动具有毒性等负面作用。但是,它们对植物是否具有毒性特别是是否会产生植物基因毒性至今尚不清楚。因此,本文使用随机扩增多态性DNA技术研究TiO2纳米颗粒是否对西葫芦具有基因毒性,为TiO2纳米颗粒排放进入环境后的潜在植物毒性风险评价提供依据。创新要点:首次发现了TiO2纳米颗粒对西葫芦具有基因毒性。重要结论:采用随机扩增多态性DNA技术,发现TiO2纳米颗粒污染处理的西葫芦样品与未处理样品的基因组DNA图谱相比,不仅在谱带强度有明显差异,而且存在谱带消失和新谱带产生现象,表明TiO2纳米颗粒对西葫芦具有基因毒性。Titanium dioxide nanoparticles （TiO2 NPs） are used in cosmetics, stmscreens, paints, and toothpaste, among other applications. These NPs are very stable and can be transported and dispersed in wastewater and biosolids. Animal species have shown negative reactions to TiO2 NPs. However, little is known about their toxicity in plants, specifically the possibility of gen- otoxic effects. In this study, we used a random amplified polymorphic DNA （RAPD） technique to study the genotoxic effects of TiO2 NPs on hydroponically cultivated zucchini （Cucurbitapepo） plants. Seeds were allowed to germinate for 7 d and plants were selected at random for individual and population studies. Four plants were selected for the individual study and 18 for the popu- lation study. RAPD profiles of TiO2 NPs treated plants showed differences in band intensity, loss of bands, or appearance of new bands, compared to untreated plants. To the authors＇ knowledge, this is the first report of the genotoxic potential of TiO2 NPs in zucchini.

关 键 词：随机扩增多态性DNA技术 TIO2 纳米颗粒 基因组DNA 西葫芦 毒性 

分 类 号：X173[环境科学与工程—环境科学]