HPLC同时测定调经活血片中芍药苷、阿魏酸、金丝桃苷的含量  

Silmultaneous Determination of Paeniflorin,Ferulic Acid and Hyperoside in Tiaojing Huoxue Pills by HPLC

作　　者：谢媛[1,2] 涂瑶生 施之琪 周蓉[1,2] 

机构地区：[1]广州中医药大学,广州510405 [2]广东省中医药工程技术研究院,广州510095

出　　处：《中国实验方剂学杂志》2014年第17期84-87,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的：采用HPLC同时测定调经活血片中芍药苷、阿魏酸、金丝桃苷的含量。方法：采用Elipse-C18色谱柱（4.6 mm×150 mm,5μm）,流动相乙腈-0.1%磷酸（14∶86）,流速1.00 mL·min^-1,柱温25℃,检测器DAD,检测波长230 nm（芍药苷）,316 nm（阿魏酸）,360 nm（金丝桃苷）。结果：芍药苷在160.0～1280.0μg与峰面积呈良好的线性关系（r=0.999 6）,平均回收率为98.38%,RSD1.51%;阿魏酸在67.5～540.0μg与峰面积呈良好的线性关系（r=0.999 3）,平均回收率为98.33%,RSD1.21%;金丝桃苷在17.2～137.6μg与峰面积呈良好的线性关系（r=0.999 8）,平均回收率98.71%,RSD1.27%。结论：该方法简便、灵敏、准确、重复性好,可用于调经活血片的质量控制。Objective： The study was aimed to determine the contents of paeniflorin,ferulic acid and hyperoside in Tiaojing Huoxue pills by HPLC. Method： The chromatographic column was Elipse-C18（4.6 mm ×150 mm,5 μm） with acetonitrile-0. 1% phosphoric acid（14∶ 86） as mobile phase. The flow rate was fixed at1. 0 mL ·min^-1,the column temperature was maintained 25 ℃ and the detector was DAD. The detection wavelengths were set at 230 nm,316 nm for paeniflorin,ferulic acid and hyperoside,respectively,the detection wavelength of hyperoside was set at 360 nm. Result： There is good linear relationship within the range of 160. 0-1280. 0 μg for paeniflorin,67. 5-540. 0 μg for ferulic acid,and 17. 2-137. 6 μg for hyperoside. The average revcoveries of paeniflorin,ferulic acid and hyperoside were 98. 38%（RSD of 1. 51%）,98. 33%（RSD of1. 21%） and 98. 71%（RSD of 1. 27%） respectively. Conclusion： This method is simple, accurate and reproducible. It can be used for the quality control of Tiaojing Huoxue pills.

关 键 词：调经活血片 芍药苷 阿魏酸 金丝桃苷 高效液相色谱 

分 类 号：R284.1[医药卫生—中药学]