血管内皮生长因子和缺氧诱导因子-2在良、恶性嗜铬细胞瘤组织中的表达及意义  

Expression and significance of the vascular endothelial growth factor and hypoxia induced factor-2 in benign and malignant adrenal pheochromocytoma

作　　者：李烨[1] 罗海红[1] 杨艳丽[2] 段建敏[1] 包军胜[1] 秦大山[1] 米军[1] 史庭凯[1] 卢建中[1] 马宝良[1] 

机构地区：[1]兰州大学第二医院泌尿外科甘肃省泌尿系疾病临床医学中心甘肃省泌尿系疾病研究重点实验室,730030 [2]兰州军区兰州总医院病理科

出　　处：《中华泌尿外科杂志》2014年第8期621-624,共4页Chinese Journal of Urology

摘　　要：目的 研究血管内皮生长因子（VEGF）和缺氧诱导因子-2（HIF-2）在良、恶性肾上腺嗜铬细胞瘤组织中的表达及意义.方法 选择1998-2008年经临床和病理检查证实的良性肾上腺嗜铬细胞瘤石蜡标本（20例）和冷冻标本（4例）,11例经临床及病理证实有肺或淋巴结转移的恶性嗜铬细胞瘤石蜡标本,以及11例正常肾上腺组织.采用免疫组化和原位杂交方法检测上述组织中的VEGF和HIF-2表达情况.结果 免疫组化和原位杂交检测结果一致,均发现VEGF、HIF-2在瘤细胞胞质表达,在肿瘤坏死灶周边及血管内皮细胞表达较高.正常肾上腺组织中VEGF、HIF-2阴性或可疑阳性表达;良性嗜铬细胞瘤组织中,VEGF和HIF-2为阴性至弱阳性表达,与正常组织表达差异均无统计学意义（P＞0.05）;恶性嗜铬细胞瘤组织中,VEGF、HIF-2均呈阳性、强阳性表达,与正常组织和良性嗜铬细胞瘤组织比较差异均有统计学意义（P＜0.05）.结论 VEGF、HIF-2在良、恶性嗜铬细胞瘤组织中的表达差异有统计学意义,联合检查在鉴别诊断中可能有指导意义.Objective To study the expression and significance of the vascular endothelial growth factor （VEGF） and hypoxia induced factor-2 （HIF-2） in benign and malignant adrenal pheochromocytomas.Methods Specimens of 11 normal adrenal glands,24 benign adrenal pheochromocytomas and 11 malignant pheochromocytomas were selected from 1998 to 2008.The expression of VEGF and HIF-2 was detected by immunohistochemistry and in situ hybridization.Results The same results were observed by immunohistochemistry and in situ hybridization.The expressions of VEGF and HIF-2 were negative or doubtful positive in normal adrenal tissues and negative or only weakly positive in benign adrenal pheochromocytomas,with no significant difference between normal adrenal tissue and benign adrenal pheochromocytoma （P＞0.05）.The expressions of VEGF and HIF-2 were positive or strong positive in all malignant pheochromocytomas,with significant difference contrast to normal group and benign adrenal pheochromocytomas （P ＜ 0.05）.Conclusions mRNA transcription and protein expression of VEGF and HIF-2 are significantly higher in malignant pheochromocytoma tissue than those in benign adrenal pheochromocytoma tissue and normal tissue.The co-detection of VEGF and HIF-2 may be helpful to distinguish benign and malignant pheochromocytomas.

关 键 词：嗜铬细胞瘤 肾上腺肿瘤 血管生成 血管内皮生长因子 缺氧诱导因子-2 

分 类 号：R736.6[医药卫生—肿瘤]