酶技术在中药黄酮类成分研究中的应用  

Application of enzyme technology in flavonoid ingredients of Chinese materia medica

作　　者：高霞[1,2] 刘聪燕[2] 陈彦[2] 王莹[2] 周静[2] 瞿鼎[2] 

机构地区：[1]南京中医药大学附属中西医结合医院,江苏南京210028 [2]中国中医科学院江苏分院,江苏南京210028

出　　处：《中草药》2014年第16期2412-2417,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81173557);江苏省中医药领军人才项目(LJ200913);江苏省"333人才培养工程"项目(BRA2011219);中央级公益性科研院所基本科研业务费专项资金资助

摘　　要：近年来,酶技术在中药黄酮类成分研究中的应用越来越广泛,查阅国内外文献后发现酶技术在中药黄酮类成分研究中的应用主要体现在3个方面:(1)单独应用或与其他技术联合应用(包括酶解-超声偶联技术和酶解-微波偶联技术等)于中药黄酮类成分的提取,使其提取率明显提高;(2)改变黄酮类成分的结构使其转化成活性更好、生物利用度更高的有效成分;(3)促进植物体内黄酮类成分的合成。此外,固定化酶技术在黄酮类成分中的应用也越来越多。酶技术作为中药黄酮类成分研究中的一种新方法,有助于从复杂的中药中获得更多活性较好的黄酮类成分,具有较好的应用前景,但也存在一些问题,需要更深入的研究。In recent years, enzyme technology is widely applied in the flavonoid ingredients of Chinese materia medica （CMM）. Based on a large number of domestic and foreign literatures, the application of enzyme technology in the flavonoid ingredients is mainly embodied in three aspects： significantly improving the extraction rate of flavonoid ingredients by using alone or combined with other technology including enzyme-ultrasonic coupling technology and enzyme-microwave coupling technology, obtaining the effective ingredients with a better activity and higher bioavailability by changing the structure of some flavonoid ingredients, and increasing the active flavonoids content in the plants. Moreover, the immobilized enzyme technology is applied more and more. Enzyme technology, a new method in the study on flavonoid ingredients in CMM, can help to obtain more active flavonoids from the complex samples of CMM. Therefore, the enzyme technology has a good application prospect but also with some key problems to be further studied in future.

关 键 词：酶技术 中药 黄酮类成分 结构转化 酶解-超声偶联技术 酶解-微波偶联技术 

分 类 号：R284[医药卫生—中药学]