氧化型低密度脂蛋白提高小鼠骨髓间充质干细胞粘附力  

Oxidized Low Density Lipoprotein Increases Adhesive Capacity of Mouse Bone Marrow-Derived Mesenchymal Stem Cells

作　　者：武俊芳[1] 牛杰[1] 李晓鹏[2] 张芬熙[3,4] 高利洁[1] 崔柳苏[1] 闫晓晓[1] 

机构地区：[1]新乡医学院基础医学院形态学实验室,河南新乡453003 [2]新乡医学院第三附属医院眼科,河南新乡453003 [3]新乡医学院三全学院解剖教研室,河南新乡453003 [4]新乡医学院干细胞与生物治疗研究中心,河南新乡453003

出　　处：《河南大学学报（自然科学版）》2014年第5期582-585,共4页Journal of Henan University:Natural Science

基　　金：河南省教育厅自然科学基金资助项目(2010A310006)

摘　　要：分别采用5、10和20μg/mL的氧化型低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)处理小鼠骨髓间充质干细胞(bmMSCs)6h,通过CellTracker预染小鼠单核细胞,检测单核与bmMSCs之间的粘附率,研究ox-LDL对bmMSCs粘附的影响.利用Western blot检测粘附分子VCAM-1和ICAM-1于ox-LDL处理后在bmMSCs内的表达情况.结果显示:bmMSCs阳性表达间充质干细胞特异性标记分子CD44和CD90;ox-LDL可显著提高bmMSC与单核细胞之间的粘附(P<0.05),增强粘附分子VCAM-1和ICAM-1的表达(P<0.05),且具有剂量效应.以上结果表明,ox-LDL通过增强粘附分子VCAM-1和ICAM-1的表达进而增强bmMSCs对单核细胞的粘附,其或许可作为一种提高间充质干细胞粘附和提高干细胞移植后存活率的诱导剂.To study the effect of oxidized low-density lipoprotein （ox-LDL） on adhesion of mouse bone marrow mesenchymal stem cells （bmMSCs）, BmMSCs were treated with 5, 10 and 20μg/mL ox-LDL for 6 h. Adhesive rate of mouse monocy＇tes on bmMSCs was measured through pre-dye of monocytes with CellTracker. Expression of adhesive molecules VCAM-1 and ICAM-1 in bmMSCs were measured by Western blot assay after treatments with ox-LDL. BmMSCs were positive for MSC specific makers CD44 and CD90. Treatments with ox-LDL markedly increased adhesive rate of monocytes on bmMSCs, and enhanced expression of VCAM1 and ICAM1 in a dose-dependent manner （ P〈0.05）. These results indicate that ox-LDL can enhance adhesion of bmMSCs to monocytes via increase of expression of adhesion molecules VCAM-1 and ICAM-1, and maybe serve as an inducer to increase adhesion and survivability of MSCs after transplant.

关 键 词：氧化型低密度脂蛋白 骨髓间充质干细胞 细胞粘附 粘附分子 

分 类 号：R73-35[医药卫生—肿瘤]