油水乳化体系中分枝杆菌转化植物甾醇产9α-羟基雄甾烯酮工艺研究  

Biotransformation of Phytosterols to 9α-Hydroxyandrostenedione by Mycobacteriumsp.in Oil-Water Emulsion System

作　　者：高兴强[1] 冯建勋[1] 花强[1] 王学东[1] 

机构地区：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2014年第4期433-437,共5页Journal of East China University of Science and Technology

基　　金：国家自然科学基金(21276083)

摘　　要：为了提高分枝杆菌转化植物甾醇产9α-羟基雄甾-4-烯-3,17-酮(9-OH-AD)的转化效率,建立了油水乳化体系。结果表明:机械搅拌提供剪切力和加入吐温80作为分散剂是形成稳定乳化体系的必要因素,且油的比例须随甾醇投料质量浓度的增加而增加。在油水乳化体系中,细胞均匀分散,生物量增加,植物甾醇投料浓度和转化效率均得到提高;植物甾醇投料质量浓度可达20g/L,9-OH-AD摩尔得率达到64.06%,产率为1.28g/(L·d)。转化终止后通过离心进行相分离,可以去除大部分存在于油相中的副产物,使产物纯度从66%提高到90%。A simple oil-water emulsion system was developed for highly efficient biotransformation of phytosterols to 9α-hydroxyandrost-4-ene 3, 17-dione （9-OH-AD）. Results indicated that mechanical shearing force generating shear force and addition of Tween 80 as dispersant were the essential factors to form a stable emulsion system. And the ratio of cereal oil was related to the effective load of system for phytosterols concentration. In the emulsion system, a higher biomass was got at an evenly dispersed state. Application of the emulsion system accelerated bio＇transformation process at higher phyosterols concentration of 20 g/I. phytosterols with 64.06% molar yield and 1.28 g/（L·d） productivity of 9-OH AD. After biotransformation, the major by-products partitioned in the oil phase would be removed with oil, and the purity of 9 OH-AD by the chromatography area normalizing increased from 66% to 90%.

关 键 词：分枝杆菌 植物甾醇 9-羟基雄甾-4-烯-3 17-二酮 油水乳化体系 

分 类 号：TQ92[轻工技术与工程—发酵工程]