结直肠癌有无肝转移血清差异蛋白质表达的研究  

A Research Study of the Serum Proteomics Expressions on Liver Metastases Value in Colorectal Carcinoma

作　　者：张伟斌[1] 陈丹[1] 马伟钦[1] 何兴祥[1] 陈羽[1] 王丽京[2] 

机构地区：[1]广东药学院附属第一医院,广东广州510080 [2]广东药学院血管生物学研究所

出　　处：《中国医学创新》2014年第21期1-6,共6页Medical Innovation of China

基　　金：国家自然科学基金(61201437);广东省医学科研基金项目(A2011315);广东省高等学校高层次人才项目(粤教师函[2010]79号)

摘　　要：目的:利用蛋白质组学技术分离、鉴定结直肠癌有无肝转移患者血清中差异蛋白质,筛选诊断肝转移的血清蛋白标志物。方法:根据入组条件,收集结直肠癌无肝转移病例、有肝转移病例,在两组中随机抽取12例血清样本,同组血清等量混合进行双向凝胶电泳,建立两组血清蛋白质双向电泳图谱,用Image-Master V5.0软件寻找两组差异蛋白质点,MALDI-TOF-MS对差异蛋白质进行鉴定,查询生物信息数据库对差异蛋白质进行初步分析。结果:两组比较差异在2倍以上蛋白质有8种,其中5个蛋白质表达上调,3个蛋白质表达下调;两组每个差异蛋白灰度体平均值比较差异均有统计学意义(P<0.05)。通过数据库搜索鉴定出7种蛋白质,上调的5个蛋白质分别是Transferrin、Complement component C9、RNA directed DNA polymerase(RNA指导的DNA合成酶)、Conserved hypothetical-protein(假定蛋白质)、SEC14L1 7 kDa protein;下调的2个蛋白质分别是Haptoglobin和Isoform 1 of Serum albumin。结论:结直肠癌有无肝转移患者血清中的蛋白质表达谱有一定的差异性,这些差异蛋白质可能成为诊断肝转移的血清标志物。Objective： To use proteomics separation method identify colorectal carcinoma patients＇ serum proteins, so as to differentiate and diagnostic the serum protein hiomarkers for liver metastases.Method： According to inclusion criterion, patients had been divided into colorectal cancer patients with liver metastases, and patients without liver metastases. Two groups were be randomized into 12 serum samples, same amount of serum proteins were mixed in each group by two-way clear gel eleetrophoresis, followed by building two sets of two-dimensional electrophoresis pattern. Image-Master V5.0 software was used to find the differences between two samples. Kam proteins were differentiated by Matrix-assisted laser desorption/ionization time of flight mass spectrometry （ MALDI-TOF-MS ） . The differentiated expressed proteins in given bioinformatics databases was analyzed.Result： The coloreetal liver metastases group and the non-colorectal liver metastases group showed that eight proteome were spotted with more than two differentials, of which five proteins were up-regulated and two were down-regulated. Each protein gray body average differences between the two groups had statistical significance （ P〈0.05 ） . Five up-regulated proteins were ： Transferrin, Complement component C9, RNA directed DNA polymerase, Conserved hypothetical - protein, SEC14L1 7 kDa protein; two down-regulated proteins were Haptoglobin and Serum albumin Isoform 1.Conclusion： Serum protein expression spectrum shows differential in colorectal carcinoma patients with liver metastases. This differential may be the diagnostic serum biomarkers for liver metastases.

关 键 词：结直肠癌 肝转移 差异蛋白质 

分 类 号：R735.3[医药卫生—肿瘤]