高效液相色谱-二极管阵列法测定保健酒中的萘普生  

Determination of Naproxen in Health Liquor Using HPLC-PAD

作　　者：郭杰标[1] 蓝献泉 肖仔君[1] 刘旺培 严晓明 何颖娟[1] 

机构地区：[1]韶关学院英东食品科学与工程学院,广东韶关512005 [2]韶关市食品药品检验所,广东韶关512026

出　　处：《现代食品科技》2014年第8期238-241,257,共5页Modern Food Science and Technology

基　　金：广东省自然科学基金项目(S2011040001362);韶关市科技计划项目(2013CX/K97)

摘　　要：近期不断发现抗炎药物被违法添加到抗风湿保健酒中增加治疗效果，这种现象对消费者健康构成成胁。萘普生是常用非甾体抗炎药物，本研究建立保健食品中违禁添加萘普生的液相检测方法。通过酸性条件氯仿和碱性水溶液两步液-液萃取，从保健酒中提取和净化蔡普生。使用Kromasil 100．5C18（250×4．6mm E74572）色谱柱，以甲醇-0．01M磷酸缓冲液（pH3．0）（75／25，V／V）流动相，流速为1．0mL／min进行分离；检测波长为231nnl。通过比对色谱峰保留值和加标实验，结合二极管阵列扫描图谱对可疑样品定性，并根据外标法峰面积定量检测萘普生含量。检品和基质成分基本实现了基线分离，最低检测限为0．5μg／mL，工作曲线的线性范围：5～80μg／mL（R2=0．9993），RSD为1．87％（n=6），加标回收率83．3～92．6％。方法准确、可靠，适用于检测保健品中违法添加的萘普生。Recently, undeclared anti-inflammatory drug were frequently exposed being adulterated into antirheumatic health liquors to promote therapeutic effect. This phenomenon poses a serious threat to public health. Naproxen is a common nonsteroidal anti-inflammatory drug. The aim of this study is to develop a liquid chromatographic method coupled with photodiode array detector for identification of illegal adulterated naproxen in healthy food. Two-step liquid-liquid extractions were employed to purify naproxen from samples using chloroform in acidic condition following aqueous solution in alkaline condition, respectively. Chromatographic separation was achieved on a Kromasil 100-5C18columm （250×4.6 mm E74572） with the mobile phase of methanol- 10ram phosphate buffer （pH 4.0） （75/25, V/F）, at a flow rate of 1.0 mL/min. The eluant was detected at 231nm. Retention time combined with standard addition result and PAD-scanning graph were applied to verify naproxen. Quantitation of naproxen was attempted by external standard method. The results showed as follows： the limit of quantitation was 0.5 μg/mL, the linear range was 5-80 μg/mL （R2= 0.9993）, RSD was 1.87% （n=6） and the recovery of naproxen from health liquor was ranging from 83.3-92.6%. The method is suitable in determination ofnaproxen as adulterants in health liquor due to its accuracy and reliability.

关 键 词：功能食品 萘普生 违法添加 液-液萃取 色谱检测 

分 类 号：O657[理学—分析化学] TS262.91[理学—化学]