虫生广布拟盘多毛孢蛋白酶、几丁质酶、脂肪酶产生水平及其毒力关系研究  

Relationships between virulence and activities of protease,chitinase and lipase produced by entomogenous Pestalotiopsis disseminata

作　　者：黄蓉[1] 吕成群[1] 黄宝灵[1] 郭丽娟[1] 姚姜铭[1] 李瑞龙 何锦华 何钰 卢强 

机构地区：[1]广西大学林学院,南宁530005 [2]玉林市森防站,广西玉林537000 [3]北流市森防站,广西北流537400

出　　处：《南方农业学报》2014年第7期1172-1177,共6页Journal of Southern Agriculture

基　　金：国家自然科学基金项目(31170617)

摘　　要：[目的]了解虫生广布拟盘多毛孢GXSTYJ03菌株侵染和杀死松突园蚧过程中酶活性变化及作用机理.[方法]通过Folin-酚法、DNS法和对硝基苯酚法分别测定含1×104、1×105、1×106、1×107和1×108个孢子/mL系列浓度的GXSTYJ03菌株孢子悬浮液产生的蛋白酶、几丁质酶和脂肪酶活力及其对松突圆蚧的致死率.[结果]GXSTYJ03菌株孢子含量越高,其产蛋白酶、几丁质酶、脂肪酶的活力也越高;蛋白酶、几丁质酶、脂肪酶的产酶高峰期和产酶活性均不相同,蛋白酶的产酶高峰期出现在发酵后第5d、几丁质酶出现在发酵后第6d、脂肪酶出现在发酵后第4～5d,GXSTYJ03菌株的蛋白酶活性远大于几丁质酶和脂肪酶活性;GXSTYJ03菌株5种孢子浓度产蛋白酶、几丁质酶、脂肪酶活性与对松突圆蚧毒力呈极显著正相关.[结论]1×107个孢子/mL的GXSTYJ03菌株孢子悬浮液是产酶及杀虫的最佳使用量.蛋白酶、几丁质酶、脂肪酶活性越高的菌株毒力越强,用于侵染和致死松突圆蚧的效果越好.[Objective]The activities of protease,chitinase and lipase produced by entomogenous Pestalotiopsis disseminata GXSTYJ03 were studied to analyze their relationships with virulence.[Method]By using Folin-phenol,DNS and Nitrophenol method,the activities of protease,chitinase and lipase produced by different spore content of entomogenous Pestalotiopsis disseminata GXSTYJ03 such as 1 ×104,1 ×105,1 ×106,1×107 and 1 × 108 spores were measured separately,and the death rate of Hemiberlesia pitysophila Takagi infested by different concentrations of entomogenous Pestalotiopsis disseminata was calculated.[Result]The results showed that the higher concentration of entomogenous Pestalotiopsis disseminata GXSTYJ03 spore,the greater activities of protease,chitinase and lipase.The peak of protease enzyme production was on Day 5 after fermentation,the peak of chitinase enzyme production was on Day 6,and the peak of lipase enzyme production was on Day 4-5.The activity of protease was much larger than that of chitinase and lipase.In general,there were extremely significant positive correlations between the virulence and activities of protease,chitinase and lipase produced by 5 spore content of entomogenous Pestalotiopsis disseminate GXSTYJ03.[Conclusion] 1 ×107 is the best concentration of entomogenous Pestalotiopsis disseminata GXSTYJ03 for generating enzyme and killing pests.The higher activities of protease,chitinase and lipase result in the stronger strains virulence,and the effect on the Hemiberlesia pitysophila Takagi infection is also better.

关 键 词：虫生广布拟盘多毛孢 GXSTYJ03菌株 蛋白酶 几丁质酶 脂肪酶 松突圆蚧 毒力 

分 类 号：S763.306.4[农业科学—森林保护学]