大孔树脂对甜菊糖的纯化  

Purification of stevioside ST and RA bymacroporous resin

作　　者：阳小宇[1] 陈志良[2] 郝再彬[2] 

机构地区：[1]湖南化工职业技术学院,湖南株洲412004 [2]桂林理工大学化学与生物工程学院,广西桂林541004

出　　处：《广西植物》2014年第4期525-529,共5页Guihaia

基　　金：湖南省高等学校科研项目(11C0465);湖南省科技厅项目(2012SK3180)

摘　　要：通过静态吸附比较三种大孔树脂对甜菊糖中莱鲍迪甙A(RA)和甜菊甙(ST)的吸附和解吸能力,利用高效液相色谱对结果进行检测,从中筛选出对RA和ST吸附效果最佳的LX-68M树脂。纯化工艺为30℃下,LX-68M树脂对RA和ST在6h后达到吸附饱和,吸附量分别为干树脂RA 85.00mg/g和ST 121.50mg/g。动态洗脱结果表明:以55%的乙醇为洗脱液,在2BV/h的洗脱速度下,经LX-68M树脂处理后的甜菊糖溶液纯度由75.5%提高到77.8%。表明LX-68M树脂对于甜叶菊糖苷工业生产的应用前景较好。The adsorption and desorption capacities of three kinds of macroporous resins for RA and ST contained in stevioside were investigated through static adsorption experiment.The adsorption and desorption capacities was quan-tified using high performance liquid chromatography （HPLC）,and the results indicated that the LX-68M had the best adsorption and desorption capacities.Under 30 ℃,the absorption equilibrium of LX-68M resin was achieved for RA （85.0 mg/g dry resin）and ST （121.5 mg/g dry resin）after 6 h,respectively.The dynamic desorption was carried out under 2 BV/h eluting employing 55% （V%）ethanol/water as eluent,and the results showed that the purity of stevia glycosides increased from 75.5% to 77.8%.On the basis of these findings,it was believed that the LX-68M resin had industrial application promise for stevia glycosides.

关 键 词：大孔树脂 莱鲍迪甙-A 甜菊甙 分离纯化 高效液相色谱 

分 类 号：TQ461[化学工程—制药化工]