黑李溃疡病病原菌的分离与鉴定  

Isolation and identification of the pathogen of black plum canker

作　　者：姜聪[1] 张青 姚忠华 许志刚[3] 楼兵干[1] 

机构地区：[1]浙江大学生物技术研究所,浙江杭州310058 [2]临安市农业技术推广中一心,浙江临安311300 [3]南京农业大学植物保护学院,江苏南京210095

出　　处：《浙江农业学报》2014年第4期971-975,共5页Acta Agriculturae Zhejiangensis

基　　金：浙江省“三农六方”资助项目(N20120623)

摘　　要：黑李溃疡病为近年来发生在浙江省黑李产区的一种严重病害,主要危害李树当年生、多年生枝和主干,引起枝条枯死和整株死亡,继而毁灭整个黑李果园。通过对黑李溃疡病典型症状样本的采集、病原菌的分离、纯化和致病性测定,明确引起该病的病原菌为一种细菌。通过对黑李溃疡病菌的菌体形态和培养特性观察,生理生化反应、脂肪酸分析、16S rDNA序列分析和种的特异性引物的检测分析,证明该病原细菌为树生黄单胞菌李变种Xanthomonas arboricola pv.pruni(Smith)Vauterin et al.。Black plum （ Prunus americana） canker is a serious disease occurring in recent years in black plum plantation in Zhejiang Province .It mainly infects current-year shoots , perennial branches and trunks , causing the branches withered and the whole plant died , and finally destroyed the whole black plum orchard .In this study, we collected the typical symptom samples of this disease , isolated and purified the pathogen , and then the pathogenicity determination results showed that the pathogen causing black pulm canker was a kind of bacteria .The pathogenic bacteria was identified as Xanthomonas arboricola pv.Pruni（smith） Vauterin et al.based on the bacteria cell morphology , cultural characteristics , physiological and biochemical characteristics , analysis of fatty acid methylesters （FAME） and 16S rDNA gene sequence.

关 键 词：病原菌鉴定 黑李溃疡病 脂肪酸分析 树生黄单胞菌李变种 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]