柱前衍生RP-HPLC测定淫羊藿中氨基酸含量  

Determination of amino acids in Epimedium brevicornu Maxim. by RP-HPLC

作　　者：赵岩[1] 侯莹莹[1] 唐国胜[1] 王士杰[2] 蔡恩博[1] 张连学[1] 

机构地区：[1]吉林农业大学中药材学院,长春130118 [2]吉林农业科技学院中药学院,吉林132101

出　　处：《药物分析杂志》2014年第8期1412-1416,共5页Chinese Journal of Pharmaceutical Analysis

基　　金：国家科技支撑计划项目(2011BAI03B01);国家公益性行业科研专项(201303111);吉林省科技发展计划项目(20126046;20140204013YY);吉林省中医药产业发展专项(YYZX201258)

摘　　要：目的：建立柱前衍生-反相高效液相色谱法检测淫羊藿中游离氨基酸和水解氨基酸的含量。方法：采用高效液相色谱法,以异硫氰酸苯酯进行柱前衍生,梯度洗脱的方法,检测淫羊藿中游离氨基酸和水解氨基酸的含量。结果：21种氨基酸的检测浓度线性范围为0.0019~2.7000μmol·mL-1,r均≥0.9995。21种游离氨基酸的平均加样回收率在86.7%~100.1%之间,RSD在0.61%~2.7%之间（n=6）;水解氨基酸的平均加样回收率在90.3%~100.0%之间,RSD在0.41%~2.0%之间（n=6）。结论：该方法经方法学验证,适合淫羊藿中游离氨基酸和水解氨基酸的含量测定。Objective： To establish a method for determination of the contents of 21 free and hydrolyzed amino acids from Epimedium brevicornu Maxim. by pre- column derivatization RP- HPLC. Methods： The samples were derivated with phenyl isothiocyanate and HPLC method was adopted. Gradient elution chromatography was applied to determine contents of free and hydrolyzed amino acids in E. brevicornu. Results： The 21 amino acids had good linearity in the range of 0. 0019- 2. 7000 μmol·mL- 1（ the coefficients of correlation were all over 0.9995）.The average recoveries of free amino acids were 86. 7%- 100. 1%（ RSD = 0. 61%- 2. 7%,n = 6）; the average recoveries of hydrolyzed amino acids were 90. 3%- 100. 0%（ RSD = 0. 41%- 2. 0%,n = 6）. Conclusion： The methodology validation showed that the method was suitable for determination of free and hydrolyzed amino acids in E. brevicornu.

关 键 词：淫羊藿 氨基酸 异硫氰酸苯酯 柱前衍生化 高效液相色谱法 中药含量测定 

分 类 号：R917[医药卫生—药物分析学]