hTERT对干预后人胚胎神经元cdk5 P-CREB表达影响的初步观察  

Influence of hTERT on the expressions of cdk5,P-CREB of human embryonic cerebral cortex neuron after intervened with Aβ25-35

作　　者：吴灵芝[1] 李水彬[1] 成钢卫[1] 汪华侨[2] 孔令平[3] 

机构地区：[1]中山大学附属梅州医院神经内二科,梅州514031 [2]中山大学中山医学院解剖学教研室脑研究室,广州510080 [3]广州医科大学卫生职业技术学院,广州510925

出　　处：《中国实用神经疾病杂志》2014年第16期1-3,共3页Chinese Journal of Practical Nervous Diseases

基　　金：广东省教育厅科技创新项目(2012KJCX0089)

摘　　要：目的对干预后人胚胎神经元周期素依赖性蛋白激酶5(cyclin-dependent kinase 5,cdk5)、cAMP反应元件结合蛋白(cAMP response element binding protein,P-CREB)表达影响。方法人胚胎大脑皮质神经元原代培养后,用hTERT重组腺病毒转染神经元,用Aβ25-35干预转染后神经元。实验分为转染组和非转染组。Western-blot检测cdk5、p-CREB的变化。结果 Aβ25-35干预后,神经元内cdk5表达量明显增加,而hTERT基因转染组cdk5增加的低于非转染组。干预后神经元内p-CREB表达量明显降低,而hTERT基因转染组,p-CREB表达量高于非转染组。结论 Aβ25-35干预人胚胎大脑皮质神经元,可以导致cdk5的异常活化,p-CREB的降低;hTERT基因转染可有效抑制cdk5的异常激活,防止p-CREB的降低。Objective To observe the effects of hTERT on cdk5,P-CREB and P16 of human embryonic cerebral cortex neuron after Aβinvention.Methods Primary cerebral cortex neurons of human embryo cultured were transfected with hTERT.The neurons were dealt with Aβ25-35.Two groups were divided into the transfected group and the non-transfected group.The expressions of cdk5,p-CREB were measured by Western-blot.Results The expression of cdk5 was obviously up-regulated after the intervention of Aβ25-35,but in the group transfected with pSU-CMV-hTERT,the expression of cdk5 was decreased than that in non-transfected group.The expression of p-CREB was obviously down-regulated after the intervention of Aβ25-35,but in the group transfected with pSU-CMV-hTERT,the express of p-CREB was higher than that in the non-transfected group.Conclusion Intervention on human embryonic cerebral cortex neuron with Aβmay lead to abnormal activation of cdk5 and decrease of p-CREB.However,the hTERT transfection could inhibit abnormal activation of cdk5 and the decrease of pCREB.

关 键 词：人端粒酶催化亚基(hTERT) 人胚胎大脑皮质神经元 阿尔茨海默病 神经元周期素依赖性蛋白激酶5 CAMP反应元件结合蛋白 

分 类 号：R-33[医药卫生]