VEGFR-3和podoplanin在结直肠癌中的表达及淋巴管生成和转移中的作用  

Expression of VEGFR-3 and podoplanin in colorectal carcinoma and the role in lymphangiogenesis and lymphatic metastasis

作　　者：于欢[1] 赖登攀[1] 徐立群[1] 任晓华[1] 

机构地区：[1]杭州师范大学附属医院急诊科,浙江杭州310015

出　　处：《全科医学临床与教育》2014年第4期367-370,F0002,共5页Clinical Education of General Practice

摘　　要：目的观察血管内皮生长因子受体3(VEGFR-3)及肾小球足突细胞膜黏蛋白(podoplanin)在结直肠癌组织中的表达,探讨VEGFR-3及podoplanin在结直肠癌淋巴管生成及转移中的作用。方法应用免疫组织化学法检测52例结直肠癌组织、20例大肠息肉组织和20例正常对照组织中VEGFR-3及podoplanin的表达,分析其与结直肠癌患者的临床病理参数的相关性。结果 VEGFR-3主要表达在结直肠癌细胞胞膜及胞质中,阳性表达率67.30%,明显高于在大肠息肉组织和正常大肠组织中的阳性表达率(χ2分别=6.75、16.77,P均<0.05);其在小淋巴管内皮的阳性表达率仅为9.61%。podoplanin主要表达在癌细胞淋巴管内皮细胞上,podoplanin标记的淋巴管密度(LMVD)明显高于大肠息肉组织和正常大肠组织(t分别=3.15、3.54,P均<0.05)。VEGFR-3、podo planin标记的LMVD的表达与Dukes分期、淋巴管转移有关(χ2分别=12.63、13.26,t分别=3.57、3.02,P均<0.05)。结论 VEGFR-3很少在癌细胞淋巴管内皮表达,VEGFR-3作为淋巴管标记物的依据不足;podoplanin在结直肠癌淋巴管内皮细胞高度表达,可以作为结直肠癌淋巴管标记物。Objective To observe the expression of VEGFR-3 and podoplanin in colorectal carcinoma and investigate the role of vascular endothelial growth factor receptor 3 （VEGFR-3）and podoplanin in lymphangiogenesis and lymphatic metastasis of colorectal carcinoma. Methods The expression of VEGFR-3 and podoplanin in specimens of 52 colorectal carcinoma, 20 colorectal polyps tissues and 20 colorectal normal tissues were detected by immunohistochemistry. And the relationships between VEGFR-3, podoplanin and clinicopathological features of colorectal carcinoma were analyzed. Results VEGFR-3 was mainly expressed in colorectal carcinoma cell membrane and cytoplasm, the positive rate were 67.30% which were significantly higher than those of the colorectal polyps tissues and the normal bowel tissues （χ2=6.75,16.77,P〈0.05）. It was marginally expressed in the small lymphatic endothelium, the positive rate was 9.61%. podoplanin was mainly expressed in lymphatic endothelial cells. The lymphatic vessel density （LMVD） marked by podoplanin of colorectal carcinoma were significantly higher than those of the colorectal polyps tissues and the normal bow-el tissues （t=3.15,3.54,P〈0.05）. The expression of VEGFR-3 and podoplanin in colorectal carcinoma was significantly related with Dukes＆#39;stage and lymph node metastasis（χ2=12.63,13.26;t=3.57,3.02;P〈0.05）. Conclusion VEGFR-3 was rarely expressed in colorectal cancer lymphatic endothelium. VEGFR-3 as the basis of lymphatic marker has not adequate proofs. podoplanin that express high specificity in colorectal carcinoma can be used to mark lymphatic endothelial cells.

关 键 词：结直肠癌 血管内皮生长因子受体3 肾小球足突细胞膜黏蛋白 vascular ENDOTHELIAL growth factor receptor 3 

分 类 号：R735.34[医药卫生—肿瘤]