高效液相色谱法同时检测黑茶中16种多环芳烃化合物  

Simultaneous Determination of 16 Polycyclic Aromatic Hydrocarbons in Dark Tea by HPLC

作　　者：胡琳玲[1] 刘遵莹 刘秋玲[1] 丰金玉 秦昱[1] 肖文军[1,2] 

机构地区：[1]湖南农业大学园艺园林学院茶学系,湖南长沙410128 [2]湖南农业大学国家植物功能成分工程技术研究中心,湖南长沙410128

出　　处：《茶叶科学》2014年第4期324-330,共7页Journal of Tea Science

基　　金：国家"十二五"支撑计划资助项目(2011BAD10B00;2012BAD33B11)

摘　　要：利用高效液相色谱法建立了同时检测黑茶中16种多环芳烃化合物的方法。茶叶经丙酮和二氯甲烷超声提取、硅胶柱层析、正己烷和二氯甲烷混合洗脱。采用 C18色谱柱，以甲醇和超纯水作流动相梯度洗脱，流速0.6 mL·min^-1、柱温30℃、紫外检测波长274 nm，16种多环芳烃分离效果显著，相关系数均≥0.996，最低检出限为0.1~1.0μg·L^-1，线性范围为1.0~200.00μg·L^-1，样品加标回收率为81.9%~116.5%，相对标准偏差值均≤2%。该方法具有快速、灵敏、准确、成本低的特点，同时也降低了对实验仪器的要求，可用于同时检测分析黑茶中16种多环芳烃化合物。A high performance liquid chromatography method that can rapidly detect 16 polycyclic aromatic hydrocarbons in dark tea was established. PAHs were ultrasonic extracted by acetone and methylene dichloride from tea, and purified by silica gel column and eluted with n-hexane. The results showed that the method was satisfactory when the C18 column（4.6 mm×250 mm, 5μm） was used for HPLC, methyl alcohol and ultrapure water was used for mobile phase, the flow rate was set at 0.6 mL·min^-1, the column temperature was set at 30℃, the UV detection wavelength was set at 274 nm, 16 polycyclic aromatic hydrocarbons can be well separated, the correlation coefficient of 16 PAHs was more than 0.996, detection limit was 0.1-1.0μg·L^-1, the detection linearity range was 1.0-200.00μg·L^-1, the recovery of sample with standard was 81.9%-116.5%, the relative standard deviation was less than 2%. The method was characterized by speedily, sensitive, accuracy and low cost, it reduces the requirement for experimental instrument, and can be used for the detection of 16 polycyclic aromatic hydrocarbon in dark tea.

关 键 词：黑茶 多环芳烃 高效液相色谱 线性关系 加标回收 

分 类 号：O657.72[理学—分析化学] TS272.7[理学—化学]