黄嘌呤和尿酸在修饰电极上的电化学行为及测定研究  

Study on Electrochemical Behaviors and Determination of Xanthine and Uric Acid on Modified Glassy Carbon Electrode

作　　者：孙凯[1] 孙登明[1] 

机构地区：[1]淮北师范大学化学与材料科学学院,淮北235000

出　　处：《分析化学》2014年第7期991-996,共6页Chinese Journal of Analytical Chemistry

基　　金：安徽省高校省级自然科学研究重点项目基金(No.KJ2011A255)资助~~

摘　　要：用循环伏安法制备了银掺杂聚L-赖氨酸修饰玻碳电极,并对修饰电极表面进行了表征,研究了黄嘌呤和尿酸在该修饰电极上的电化学行为,建立了循环伏安法和示差脉冲伏安法同时测定黄嘌呤和尿酸的新方法。研究表明,修饰电极对尿酸和黄嘌呤的氧化具有较好的电催化活性,在pH 3.0磷酸盐缓冲溶液中,黄嘌呤和尿酸在银掺杂聚L-赖氨酸修饰电极上的氧化峰电位分别为0.980和0.600 V,两者的峰电位差达0.380 V。在最优实验条件下,用差分脉冲伏安法同时测定黄嘌呤和尿酸的线性范围为1.0×10-6~2.5×10-4 mol/L,检出限为5.0×10-7 mol/L。用于人尿样中黄嘌呤和尿酸的测定,获得满意结果。The silver doped poly （ L-lysine ） modified glassy carbon electrode was fabricated by cyclic voltammetry, the surface of the electrode was characterized by scanning electron microscopy and electrochemical impedance spectroscopy. The electrochemical behaviors and the simultaneous detection of xanthine and uric acid were studied by cyclic voltammetry and differential pulse voltammetry. The results indicated that this modified electrode exhibited excellent electrocatalytic activity towards the oxidation of xanthine and uric acid. The stable oxidation peaks of xanthine and uric acid appeared with the peak potential of 0. 980V and 0. 600V respectively at the modified electrode in pH 3. 0 phosphate buffer solution. The oxidation peaks of xanthine and uric acid were separated at 380 mV. Under the optimum conditions, the linear ranges for the determination of xanthine and uric acid were 1 . 00 × 10-6-2 . 50 × 10-4 mol/L respectively by differential pulse voltammetry. The detection limits were 5. 0×10-7mol/L. The method has been applied to the simultaneous detection of xanthine and uric acid in healthy human urine with satisfactory results.

关 键 词：银 L-赖氨酸 修饰电极 黄嘌呤 尿酸 循环伏安法 差分脉冲伏安法 

分 类 号：O657.1[理学—分析化学]