Alpha-芋螺毒素TxID合成方法的优化  

Optimization of α-Conotoxin TxID Synthesis

作　　者：吴勇[1] 朱芙蓉[1] 刘倩[1] 长孙东亭[1] 罗素兰[1] 

机构地区：[1]海南大学热带生物资源教育部重点实验室/海口市海洋药物重点实验室,海南海口570228

出　　处：《热带生物学报》2014年第2期107-110,135,共5页Journal of Tropical Biology

基　　金：国家国际科技合作专项(2011DFR31210);国家自然科学基金(41366002);长江学者和创新团队发展计划(IRT1123);海南省自然科学基金(313047);海南省社会发展科技专项(SF201328);海口市重点科技计划项目(2013-16)

摘　　要：在常规两步氧化法合成的基础上,对芋螺毒素TxID第2对二硫键形成的条件进行了优化.优化条件包括有无氮气保护、氧化试剂中碘的浓度、三氟乙酸（TFA）含量、氧化时间等多个参数,研究其对TxID氧化折叠的影响.结果表明,氮气保护有利于TxID的氧化折叠,碘浓度需5～10倍于巯基浓度,TFA含量对氧化折叠影响不明显,氧化时间约5 min为宜.The formation of the second disulfide bond of α-Conotoxin TxID was optimized based on the regular two-step oxidation synthesis.The optimal conditions influencing TxID oxidation were determined,such as iodine oxidation with or without nitrogen protection,TFA concentration,iodine concentration,reaction time,etc.The results indicated that the nitrogen protection during the iodine oxidation process facilitated TxID oxidative folding.The iodine was optimal for formation of the second disulfide bond when its concentration was five to ten times higher than that of the thiol,but the TFA concentration did not affect the oxidative folding distinctively.The optimum time for the iodine oxidation reaction was around 5 minutes.

关 键 词：α-芋螺毒素TxID 化学合成 碘氧化 优化 二硫键形成 

分 类 号：Q179.4[生物学—水生生物学]