鸡肉组织中氯丙嗪残留的HPLC-MS/MS检测方法的建立  

Determination of Chlorpromazine Residues in Chicken Muscle Tissues by High Performance Liquid Chromatography-tandem Mass Spectrometry

作　　者：顾华兵[1] 谢洁[2] 彭涛[3] 代汉慧[3] 王娉[3] 陈冬东[3] 范春林[3] 陈颖[3] 江海洋[2] 

机构地区：[1]中国农业科学院家禽研究所,江苏扬州225125 [2]中国农业大学,北京100193 [3]中国检验检疫科学研究院,北京100123

出　　处：《中国家禽》2014年第15期33-36,共4页China Poultry

基　　金：国家科技基础性工作专项(2013FY113100;2013BAD18B11)

摘　　要：本研究建立了鸡肉组织中氯丙嗪残留检测的高效液相色谱-串联质谱（HPLC-MS/MS）定量确证方法。建立的方法所用样品用酸化乙腈和乙腈饱和正己烷提取后,采用C18柱色谱分离,正离子扫描模式,分别以乙腈和5 mmol/L乙酸铵水溶液（含0.1%甲酸）、乙腈和水为流动相,梯度洗脱,电喷雾电离,多反应监测模式检测,内标法定量。该方法检测限为0.008μg/kg,定量限为0.025μg/kg,在0.025-2.5μg/kg的添加范围内,回收率为71.5%-86.7%,日内、日间变异系数均小于7.6%。The study developed a qualitative and quantitative method of high performance liquid chromatography-tandemmass spectrometry for the determination of chlorpromazine residues in chicken muscle tissues.According to this method,thesamples were extracted with acidic acetonitrile and hexane,chromatographic separation was performed on C18 columnusing acetonitrile and 5 mmol/L ammonium acetate（containing 0.1% formic acid）in positive mode and acetonitrile andwater in negative mode,the samples were treated by electrospray ionization working with multi-reaction mode,quantifiedwith internal standard.The limit of detection of this method was 0.008μg/kg,limit of quantity was 0.025μg/kg,the recoveriesrate in chicken muscles were from 71.5% to 86.7% at 0.025 to 2.5μg/kg addition levels,intra-day and inter-day variablecoefficient was low than 7.6%.

关 键 词：HPLC-MS/MS 氯丙嗪 鸡肉组织 残留 

分 类 号：S859.84[农业科学—临床兽医学]