HN12-TG共培养模式下HN12细胞的促神经突起生长作用  

The growth-promoting function of HN12 cell on neurites under the condition of coculture with trigeminal ganglion of SD rat

作　　者：陆伟[1] 俞志维[1] 张建军[1] 王丽珍[1] 季彤[1] 

机构地区：[1]上海交通大学医学院附属第九人民医院·口腔医学院口腔颌面-头颈肿瘤科,上海市口腔医学重点实验室,上海200011

出　　处：《中国口腔颌面外科杂志》2014年第4期307-310,共4页China Journal of Oral and Maxillofacial Surgery

基　　金：上海交通大学医工交叉研究基金(YG2011MS58)~~

摘　　要：目的:建立肿瘤神经新生现象的体外研究模型,探讨在体外共培养模式下,人舌鳞状细胞癌细胞系HN-12对Sprague-Dawley(SD)大鼠三叉神经节(TG)突起生长的影响。方法:无菌条件下将HN-12细胞与SD大鼠TG接种于6孔板的Matrigel胶内进行共培养,TG单独培养作为对照组。分别于培养的第24、48、72、96、120 h拍照,测量实验组和对照组TG节神经突起生成的长度,利用SPSS19.0软件包检测2组神经突长度有无显著差异。结果:连续培养5 d发现,在共培养组,HN-12细胞对神经节突起的形成和生长具有明显的促进作用,与对照组相比有显著差异。并且,实验组中神经节突起具有明显的趋肿瘤细胞生长特性。结论:舌鳞癌神经新生现象体外培养模型成功建立。HN-12细胞具有促神经节突起生长的作用。PURPOSE： To build a new in vitro study model of neoneurogenesis, to study the influence of HN12 cell on trigeminal ganglion of SD rat under the condition of coculture. METHODS： Mouse trigeminal ganglion（TG） and human oral squamous cancer cells（HN12） were co-cultured in Matrigel matrix. Control cultures consisted of oral squamous cancer cells alone and TG alone. Neurite outgrowth was quantitated with dark phase microscopy and image analysis（Optimas 6.1） at 24,48,72,96 and 120 h. SPSS 19.0 software package was used for statistical analysis. RESULTS： HN-12 cell had a strong influence on the neurites growth compared with control group after 5 days of consecutive observation.CONCLUSION： HN-12/TG coculture model was successfully built. HN-12 cell has a strong influence on the neurites growth.

关 键 词：口腔鳞状细胞癌 三叉神经节 共培养 肿瘤神经新生 

分 类 号：R739.8[医药卫生—肿瘤]