MMP-2和E-cadherin蛋白在食管黏膜病变中的表达  

Expressions of MMP-2 and E-cadherin in esophageal mucosal lesion

作　　者：刘江惠[1] 王晓旭[1] 左连富[1] 

机构地区：[1]河北医科大学第四医院肿瘤研究所,河北石家庄050011

出　　处：《癌变．畸变．突变》2014年第4期288-291,297,共5页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨MMP-2和E-cadherin蛋白在食管黏膜病变中的表达及其与食管癌的发生及转移的关系。方法:采用免疫组织化学方法对45例食管癌组织、21例不典型增生组织、24例正常组织中MMP-2和E-cadherin蛋白的表达进行检测,并分析MMP-2和E-cadherin表达与食管癌发生发展过程中各项病理指标的关系。结果:MMP-2蛋白在癌组织中的表达率为40.00%(18/45),在不典型增生组织中的表达率为38.10%(8/21),在正常组织中表达率为4.17%(1/24),三者间差异具有统计学意义(P<0.05);有淋巴结转移组的MMP-2蛋白阳性表达率(55.56%,15/27)明显高于无淋巴结转移组(16.67%,3/18);有纤维膜浸润组MMP-2蛋白的阳性表达率(48.57%,17/35)明显高于无纤维膜浸润组(10.00%,1/10)。E-cadherin蛋白在正常的食管黏膜上皮组织阳性表达为100%(24/24),在不典型增生组织中的阳性表达率为71.43%(15/21),在癌组织中的阳性表达率为28.89%(13/45),较前两组明显降低(P<0.05)。E-cadherin与MMP-2蛋白的表达呈负相关(=4.615,r=-0.793,P<0.05)。结论:MMP-2蛋白的高表达和E-cadherin蛋白的低表达可能与食管癌的发生和转移有关,在食管癌的侵袭、转移过程中MMP-2与E-cadherin有拮抗作用。To explore the relationship between the protein expressions of MMP-2 and E-cadherin and the development and metastasis of esophageal carcinoma. METHODS：The expressions of E-cadherin protein and MMP-2 protein were examined by immunohistochemistry （IHC） in 45 cases of esophageal carcinoma,21 cases of atypical hyperplasia and 24 cases of normal esophageal mucosa tissues. RESULTS：The positve rates of MMP-2 protein were 40.00%（18/45） in esophageal carcinoma,38.1%（8/21） in esophageal atypical hyperplasia and 4.17%（1/24） in normal tissues. There were significant differences among three groups （P〈0.05）. Additionally,the expression of MMP-2 protein had a correlation with lymph node metastasis （P〈0.05）. The positve rate of MMP-2 protein in esophageal cancer tissues with lymph node metastasis was 55.56% （15/27） , significantly higher than that in esophageal cancer tissues without lymph node metastasis （16.67%,3/18）. The positve rate of MMP-2 protein in esophageal cancer tissues with fibrous membrane infiltration was higher than that in esophageal cancer tissues without fibrous membrane infiltration（48.57%,17/35;10.00%,1/10）. The positive rate of E-cadherin protein in normal tissues （100%） was much higher than that in esophageal carcinoma （28.89%） and esophageal atypical hyperplasia （71.43%）. According to these results,the expression of E-cadherin protein had a negative correlation with the expression of MMP-2 protein 2（ =4.615,r=-0.793,P〈0.05）. CONCLUSION：There were significant relationships between the high expression of MMP-2 protein and low expression of E-cadherin protein with the development and metastasis of esophageal carcinoma.Furthermore,the expression of E-cadherin protein had a negative correlation with the expression of MMP-2 protein in the invasion and metastasis of esophageal carcinoma.

关 键 词：食管黏膜病变 MMP-2 E-CADHERIN 免疫组化 

分 类 号：R734.2[医药卫生—肿瘤]