ENC1与无功能垂体腺瘤侵袭性的相关性实验研究  

Identification of ENC1 gene specific to invasiveness in pituitary null cell adenomas

作　　者：冯洁[1] 洪礼传[1] 李储忠[1] 于升远 王红云[1] 何乐[1] 李丹[1] 杨晶晶[1] 万虹[1] 张亚卓[1] 

机构地区：[1]首都医科大学北京市神经外科研究所,100050

出　　处：《中华神经外科杂志》2014年第8期807-810,共4页Chinese Journal of Neurosurgery

基　　金：国家自然科学基金（81271522）

摘　　要：目的 探讨ENC1基因与无功能垂体腺瘤（NFPAs）的发生及侵袭性的相关性,并比较其在裸细胞瘤和瘤样细胞瘤这两种NFPAs亚型中表达是否存在差异.方法 选择23例裸细胞瘤、20例瘤样细胞瘤和3例正常垂体组织.采用QPCR和Western blot检测ENC1在正常垂体组织和肿瘤组织中的mRNA和蛋白表达情况;采用免疫组化检测ENC1蛋白在肿瘤中的表达情况.结果 QPCR和Western blot结果显示在裸细胞瘤和瘤样细胞瘤中ENC1的表达明显低于正常垂体组织,且ENC1在侵袭性裸细胞瘤的表达明显低于非侵袭性裸细胞瘤的表达（P＜0.0001和P＜0.005）,而ENC1在侵袭性和非侵袭性瘤样细胞瘤中的表达差异无统计学意义（P＞0.05）.免疫组化结果显示ENC1蛋白在正常垂体组织表达呈强阳性,在非侵袭性裸细胞瘤为中等表达,而在侵袭性裸细胞瘤中呈弱阳性.结论 ENC1与裸细胞瘤的侵袭性相关,而与瘤样细胞瘤的侵袭性不相关,其可能是与裸细胞瘤侵袭性特异相关的一个重要基因.Objective Non-functioning pituitary adenomas （NFPAs） may be locally invasive.Surgery is a treatment option,but unlike the case for functional pituitary adenomas,there are almost no drug treatments available for NFPAs.To identify the relationship of ENC1 gene with invasiveness in NFPAs and to observe whether the ENC1 expression level was different between invasive null cell adenomas and invasive oncocytomas.Methods In this study,we selected 23 null cell adenomas （14 invasive and 9 non-invasive tumors）,20 oncocytomas （10 invasive and 10 non-invasive tumors） and 3 normal pituitary glands.ENC1 mRNA and protein expression level in all pituitary tumors and normal pituitary glands was detected by QPCR and western-blot.ENC1 protein expression level was observed by immunohistochemistry （IHC）.Results Low levels of ENC1 were found in null cell adenomas and oncocytoma,while high levels were detected in normal pituitary glands.ENC1 mRNA expression level was reduced in invasive null cell adenomas compared to non-invasive null cell adenomas （P ＜ 0.0001） but the results were not found between invasive and non-invasive oncocytomas.Western blot analyses showed that ENC1 expression was significantly lower in invasive null cell adenomas compared with non-invasive null cell adenomas （P ＜ 0.005） and normal pituitary glands.The immunostaining results indicated the ENC1 staining was lighter in invasive null cell adenomas than in non-invasive null cell adenomas,and it was darker in normal pituitary tissues.Conclusion ENC1 may play an important role in invasive null cell adenomas.It could be a specific biomarker for invasive null cell adenomas.

关 键 词：垂体腺瘤 侵袭 基因 ENC1 

分 类 号：R736.4[医药卫生—肿瘤]