GABA对体外培养大鼠神经元GABA_A受体亚单位mRNA表达的影响  

Effect of GABA on the expression of the mRNA of GABAAreceptor subunits in cultured rat neurons

作　　者：李晓青[1] 刘鹏[1] 种莉[1] 唐鹏[1] 刘玥[1] 陈丽[1] 张欣[1] 侯辰[1] 李锐[1] 郭民侠[1] 

机构地区：[1]陕西省人民医院神经内三科,西安710068

出　　处：《脑与神经疾病杂志》2014年第4期260-263,共4页Journal of Brain and Nervous Diseases

基　　金：陕西省重点领域科技创新团队项目(2012KCT-17)

摘　　要：目的观察γ-氨基丁酸(GABA)对体外大鼠培养神经元GABAA受体亚单位mRNA表达的影响,探讨肝性脑病(HE)发病机制。方法采用原位杂交技术,观察GABA对体外培养新生大鼠神经元GABAA受体亚单位α1β1γ2mRNA表达的改变。结果与对照组相比,GABA组神经元GABAA受体亚单位α1及γ2(增幅14%,P<0.01)的mRNA表达显著上升,亚单位β1的mRNA表达显著减少(降幅11%,P<0.05)。结论GABA可通过影响GABAA受体亚单位的表达发挥其对肝性脑病的致病作用。Object ive To observe the expression of gama-aminobutyric acid A （ GABAA ） receptor subunits mRNA in vitro cultured rat neurons by gama-aminobutyric acid （ GABA ） , discuss the pathogenesis of hepatic encephalopathy （ HE） .Methods Using the hybridization in situ to observe the change of GABA A receptor subunitsα1 ,β1 and γ2 mRNA in vitro cultured rat neurons by GABA .Results In group of cultured neurons by GABA , mRNA expression levels of GABA A receptor subunits α1 ,γ2 （ increasing amplitude 14%, P〈0.05 ） increased significantly than those of control group but subunit β1（ decreasing amplitude 11%,P〈0.05） decreased significantly . Conclusion The effect of GABA on the HE could be conducted by modulating the expression of the GABA A receptor subnuit .

关 键 词：Γ-氨基丁酸 神经元培养 原位杂交 GABAA受体 

分 类 号：R741.02[医药卫生—神经病学与精神病学]