坛紫菜与Pyropia radi种间杂交重组优良品系的选育与特性分析  

Selection and characterization of an improved strain produced by genetic recombinant of interspecific hybridization between Pyropia haitanensis and Pyropia radi

作　　者：吴宏肖 严兴洪[1,2] 宋武林 刘燕飞 刘长军 王铁杆 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海201306 [3]福建省海洋经济运行监测与评估中心,福建福州350003 [4]福建省水产技术推广总站,福建福州350003 [5]浙江省象山县水产技术推广站,浙江象山315700 [6]浙江省海水养殖研究所,浙江温州325000

出　　处：《水产学报》2014年第8期1079-1088,共10页Journal of Fisheries of China

基　　金：国家"八六三"高技术研究发展计划(2012AA10A411);国家自然科学基金(31072208);农业部公益性专项(200903030);国家农业科技成果转化资金项目(2013GB2C220537);上海市科委重点科技攻关项目(10391901100);国家海洋局公益专项(201105008;201105023);福建省科技重大专项(2011NZ0001-1);浙江省重大科技专项(2012C12907-6);上海高校水产学一流学科建设项目

摘　　要：为获得具明显杂交重组优势的紫菜优良品系,实验以印度产紫菜Pyropia radi野生型品系（Pr-WT01,特点：藻体厚、生长慢、韧性好、壳孢子放散量多）为父本,坛紫菜诱变品系（Ph-HMC5,特点：藻体薄、生长快、韧性差、壳孢子放散量少）为母本进行种间杂交,从杂合丝状体产生的F1叶状体中选出4个综合性状优良的杂交重组品系,HR-6品系为其中的最优良品系。父本品系叶状体的绝对生长率最大值为0.39 cm/d,母本品系为5.24 cm/d,而HR-6品系高达9.27 cm/d,在日龄30~50 d期间,它的平均绝对生长率比母本品系的最大生长率还大。日龄60 d的叶状体平均体长父本品系为13.18 cm,母本品系为85.67 cm,而HR-6品系已达218.57 cm,分别是父、母本品系的16.58倍和2.55倍。日龄35 d的HR-6品系叶状体的叶绿素a含量高达9.43 mg/g,比父、母本品系分别增加了50%和20%;另外,它的总藻胆蛋白含量高达99.62 mg/g,分别是父、母本品系的2.18倍和1.74倍。日龄35 d的HR-6品系的叶状体平均厚度为26.22μm,比父本品系降低了37%,比母本品系增加了30%,叶状体的韧性明显增加。HR-6品系的壳孢子放散总量达167.72万个/贝,分别为父、母本品系的1.13和2.65倍。上述研究结果表明,HR-6品系具有生长快、品质好、壳孢子放散量大等优良性状,表现出良好的生产适用性。In this paper,a crossing-experiment between Pyropia radi from India and Pyropia haitanenesis from China was done for breeding of improved strains with obvious hybrid recombinant advantages. The male parent was the wild-type strain（ Pr-WT01） of P. radi,which was characterized by thick blade,slow growing,good toughness and high production of conchospores,and the female parent was the mutation strain（ Ph-HMC5） of P. haitanensis,which was characterized by thin blade,fast growing,poor toughness and low production of conchospores. At first,four improved strains with obvious hybrid recombinant advantages were obtained from the F1color-sectored blades,and finally the most improved strain（ HR-6） was selected from them. The maximum of absolute growth rate of the male and female parents were 0. 39 and 5. 24 cm /d,respectively,while that of HR-6 strain was 9. 27 cm /d and its absolute growth rate was greater than the maximum growth rate of the female parent during 30- 50 days in culture. After 60 days in culture,the mean length of F1 blades of the male and female parents were 13. 18 cm and 85. 67 cm,respectively,while that of HR-6 strain was 218. 57 cm which was 2. 55 times that of the female parent and 16. 58 times that of the male parent,respectively. The contents of Chl. a in the 35-day-old blades of HR-6 strain was 9. 43 mg /g,increasing by 50% and 20% in contrast with that of the male parent and the female parent,respectively. The content of phycobiliprotein of HR-6 strain was 99. 62 mg /g,which was 1. 74 times that of the female parent and 2. 18 times that of the male parent,respectively. The mean thickness of the 35-day-old blades of HR-6strain was 26. 22 μm,decreasing by 37% in contrast with that of the male parent and increasing by 30% in contrast with that of the female parent,and the toughness of the F1 blades of HR-6 strain increased significantly. The total number of conchospores released from HR-6 strain was 167. 72 × 104,which was2.65 times that of the female parent and 1. 13 times that of the ma

关 键 词：坛紫菜 Pyropia radi 种间杂交 重组品系 生长率 光合色素 

分 类 号：S968.4[农业科学—水产养殖]