核苷酸对大黄鱼生长性能、肠道形态和抗氧化能力的影响  

Effects of dietary nucleotides on growth performance,intestinal morphology and anti-oxidative capacities of large yellow croaker( Larimichthys crocea)

作　　者：苗新[1] 曹娟娟[1] 徐玮[1] 张文兵[1] 麦康森[1] 

机构地区：[1]中国海洋大学水产学院水产动物营养与饲料农业部重点实验室海水养殖教育部重点实验室,山东青岛266003

出　　处：《水产学报》2014年第8期1140-1148,共9页Journal of Fisheries of China

基　　金：公益性行业(农业)科研专项(201003020,200903029)

摘　　要：为探究饲料中添加核苷酸对大黄鱼生长性能、肠道形态和抗氧化能力的影响,实验选取初始体质量为（7.71±0.02）g的大黄鱼幼鱼为研究对象,设置2个对照组,即高鱼粉对照组FM（鱼粉含量为45.0%）和低鱼粉对照组N0（鱼粉含量为31.5%）,在低鱼粉对照组基础上分别添加0、75、150、300、600和1 000 mg/kg的核苷酸,共配制出7种等氮等脂饲料,在海水浮式网箱中进行为期63 d的养殖实验。结果表明：饲料中添加核苷酸对大黄鱼的存活率、饲料效率、摄食率、鱼体组成和形体指标没有显著影响（P〉0.05）;但增重率随着核苷酸添加量的增加呈先升高后下降趋势,在添加量为300~600 mg/kg时显著高于其他处理组（P〈0.05）。随着核苷酸添加量的增加,血清中超氧化物歧化酶（SOD）和总抗氧化力（T-AOC）的活力呈先升高后下降的趋势,并在300 mg/kg时显著高于低鱼粉对照组（P〈0.05）。核苷酸对大黄鱼肠道肌肉层厚度和微绒毛高度有显著影响（P〈0.05）,分别在添加量为150和300mg/kg时出现最大值;而对肠道褶皱高度无显著影响（P〉0.05）。结果表明,饲料中添加核苷酸能促进大黄鱼生长,改善肠道形态结构和增强机体的抗氧化能力。以增重率为指标,利用折线模型计算得到大黄鱼幼鱼饲料中核苷酸的适宜添加量为194.91 mg/kg。The present study was conducted to analyze the effects of dietary nucleotides on growth performance,intestinal morphology and anti-oxidative capacities of juvenile large yellow croaker（ Larimichthys crocea） [initial weight：（ 7.71 ±0.02） g]. Two control diets were set,which were high fish meal（ FM,45. 0%） supplemented diet and low fish meal（ N0,31. 5%） supplemented diet,respectively. Based on the latter,five graded levels of nucleotides were added. They were 75,150,300,600 and 1 000 mg /kg,respectively. After the 9-week growth trial,results showed that survival rate,feed efficiency,feed intake and body composition of the animals were not significantly influenced by dietary nucleotides levels（ P 〉 0. 05）.Weight gain rates（ WGR） of fish fed the diets with 300 or 600 mg /kg of nucleotides were significantly higher than those in the other groups（ P 〈 0. 05）. Dietary nucleotides significantly affected the total antioxidative capacity（ T-AOC） and superoxide dismutase（ SOD） activity in serum（ P 〈 0. 05）. Values of T-AOC and SOD increased first,and then declined as the dietary nucleotides levels increased. The highest values of these two parameters were found in the treatment with 300 mg /kg of dietary nucleotides. Dietary nucleotide significantly increased microvillus height（ HMV） and the thickness of the muscular layer（ TML） in the proximal intestine（ P 〈 0. 05）. The HMV in intestine of fish fed diet with 300 mg /kg of nucleotides was higher than that in the control group. The TML in intestine of fish fed diet with 150 mg /kg of nucleotides was higher than that in the control group. There was no significant difference in fold height（ HF） of intestines among all groups（ P 〉 0. 05）. In conclusion,dietary nucleotides can improve the growth of the large yellow croaker. Meanwhile,it can also increase the anti-oxidative capacity and improve the intestinal morphology.Based on the data of WGR,the broken-line analysis showed that the optimal level of dietar

关 键 词：大黄鱼 核苷酸 生长 抗氧化 肠道形态 

分 类 号：S963[农业科学—水产养殖]