京尼平交联L-赖氨酸修饰胶原蛋白支架的性能和生物相容性  

Properties and Biocompatibility of Collagen Scaffold Modified by Genipin Cross-linked L-lysine

作　　者：姜东林[1] 杨骏宇 姜升阳[1] 吕国忠[1] 赵朋[1] 

机构地区：[1]南通大学第三附属医院中心实验室,无锡214041 [2]江南大学药学院,无锡214122

出　　处：《生物医学工程学杂志》2014年第4期816-821,共6页Journal of Biomedical Engineering

基　　金：江苏省自然科学基金资助项目(BK2012543)

摘　　要：胶原蛋白(Coll)作为细胞生长支架基质,在再生医学和组织工程学领域得到广泛应用。本研究以L-赖氨酸(Lys)修饰胶原蛋白,并采用京尼平(GN)交联的方式,制备Lys修饰胶原蛋白(Lys-Coll-GN)支架,考察支架的微结构、孔径、孔隙率、稳定性和生物相容性。研究发现:Lys-Coll-GN支架的Lys与胶原蛋白分子间通过形成酰胺键相连,小鼠胚胎成纤维细胞在制备的Lys-Coll-GN支架上增殖未受抑制;Coll支架、京尼平交联胶原蛋白(Coll-GN)支架与Lys-Coll-GN支架的组间比较结果显示Coll支架力学性能最差且生物降解速率最高;与Coll-GN支架相比,Lys-Coll-GN支架纤维结构增多,孔隙率高,断裂拉伸力减少但断裂拉伸长度显著增加(均P<0.01);使用胶原蛋白酶降解5d后,Lys-Coll-GN支架各时间点的降解剩余质量率略有降低(均P<0.05)。实验提示,Lys-Coll-GN支架在满足对细胞良好增殖特性的同时,有效改善了Coll支架的力学性能及降解速率。本研究或可为再生医学提供一种更有优势的新型组织工程支架材料。Collagen （Coll）, as the basic material of matrix scaffolds for cell growth, has been widely used in the field of tissue engineering and regenerative medicine. In this study, collagen protein was modified by L-lysine （Lys）, and cross-linked by genipin （GN） to prepare the L-lysine-modified collagen （Lys-Coll-GN） scaffolds. Microstructure, pore size, porosity, stability and biocompatibility of Lys-Coll-GN scaffolds were observed. The results showed that the bond between L-lysine and collagen protein molecule was formed by generating amide linkage, and mouse embryo fibroblasts proliferation was not inhibited in the Lys-Coll-GN scaffolds. In the multiple comparisons of Coil-scar folds, Coll-GNscaffolds and Lys-Coll-GN-scaffolds, Coll-scaffolds was the worst in mechanical characteristics while the highest in biodegradation rate. Compared to Coll-GN scaffolds, Lys-Coll-GN scaffolds had more fiber structure, higher interval porosity （P〈0.01）. Although the tensile stress of Lys-Coll-GN scaffolds reduced significantly, its elongation length extended when the scaffolds was fractured （P〈0.01）. The percentage of Lys-Coll-GN scaffolds re sidual weight was lower than that of Coll-GN-seaffolds after all the scaffolds were treated by collagenase for 5 days （P〈0.01）. This study suggested that Lys-Coll-GN scaffold had good biocompatibility, and it improved the mechanical property and degradation velocity for collagen-based scaffold. This study gave a new predominant type of tissue engineering scaffold for the regenerative medicine.

关 键 词：胶原蛋白 L-赖氨酸 京尼平 组织工程 

分 类 号：R318.08[医药卫生—生物医学工程]