碱性成纤维细胞生长因子对全脑缺血再灌注大鼠脑皮质内源性神经干细胞的影响  

Effect of Basic Fibroblast Growth Factor on Endogenous Neural Stem Cell in Rat Cerebral Cortex with Global Cerebral Ischemia-reperfusion

作　　者：任铭新[1] 邓晓慧[1] 郭义威[1] 郑风劲[2] 冯志博[1] 

机构地区：[1]新乡医学院人体解剖学教研室,新乡453003 [2]四川大学基础医学与法医学院,成都610041

出　　处：《生物医学工程学杂志》2014年第4期846-849,共4页Journal of Biomedical Engineering

基　　金：河南省教育厅2007年立项课题资助(2007310011);2011年新乡医学院重点研究领域招标课题项目资助(ZD2011-14);四川大学校青年基金资助项目(2012SCU11900-6)

摘　　要：探讨碱性成纤维细胞生长因子(bFGF)对全脑缺血再灌注大鼠脑皮质内源性神经干细胞增殖、迁移和分化的影响。本研究以"四血管法"制作全脑缺血再灌注模型,采用免疫组化方法检测BrdU和Nestin在不同组别各时间点的表达,用RT-PCR技术半定量检测大鼠脑皮质NSE mRNA的表达。结果显示假手术组大鼠脑皮质基本未见到BrdU和Nestin阳性细胞表达,NSE mRNA表达没有变化;模型组大鼠脑皮质的BrdU和Nestin阳性细胞在全脑缺血再灌注3d后开始增加,7d达到高峰,NSE mRNA表达无明显增加;bFGF治疗组BrdU、Nestin阳性细胞较模型组在缺血各时间点均明显增加(P<0.05),11d达到高峰,NSE mRNA表达与假手术组和模型组相比第15d表达有明显增加(P<0.05)。结果表明全脑缺血再灌注能够引起内源性神经干细胞的原位增殖,bFGF可促进全脑缺血再灌注大鼠内源性神经干细胞原位增殖,并延长增殖期,而且有明显促进内源性神经干细胞向神经元分化的作用。The present paper is aimed to investigate the effect of basic fibroblast growth factor （bFGF） on proliferation, migration and differentiation of endogenous neural stem cell in rat cerebral cortex with global brain ischemiareperfusion. A global brain ischemia-reperfusion model was established. Immunohistochemistry was used to observe the pathological changes and the expression of BrdU and Nestin in cerebral cortex. RT-PCR was used to measure the NSE mRNA in brain tissue. The results of measurements indicated that in sham operation group, there was no posi tive cell in cerebral cortex, and the content of NSE mRNA did not change. In the operation group, the expression of BrdU and Nestin increased significantly at the end of the 3rd day, and peaked on the 7th day. NSE mRNA expression did not significantly increase. In bFGF group, compared with sham operation group and model group, the num bet of BrdU-positive and Nestiwpositive cells increased significantly at each time point （P〈0.05）, and peaked at the end of the llth day, and the content of NSE mRNA increased significantly （P〈0.05）. This research demonstrated that the proliferation of endogenous neural stem cells in situ could be induced by global cerebral ischemia and reperfusion, and could be promoted and extended by bFGF. In additiion, bFGF might promote endogenous neural stem cells differentiated into neurons.

关 键 词：全脑缺血 内源性神经干细胞 碱性成纤维细胞生长因子 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]