液相芯片技术在临床检验中的应用进展  

Clinical application and prospect of suspension array technology

作　　者：王文红[1] 熊御云[1] 焦志军[1] 

机构地区：[1]江苏大学附属医院检验科中心实验室,镇江212001

出　　处：《中华检验医学杂志》2014年第8期570-572,共3页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目（81172871,81373217）;镇江市卫生科技重点项目（SH2011051）

摘　　要：液相芯片技术平台为临床诊断中的多重指标分析及批量标本检测提供了自动化的检测手段,可以从少量样本中快速得到大量的辅助诊断信息,正逐步从科学研究实验室进入临床实验室,在多种肿瘤标志物联合检测、自身抗体筛查、人类白细胞抗原（HLA）分型及感染性病原体基因检测等方面具有重要的临床应用价值.但目前仍存在较多制约该技术广泛应用于临床诊断的因素,应进一步做好组合项目的开发及检测的规范化工作,充分利用这一高通量、高灵敏度和高重复性的检测平台,为临床疾病的诊断、治疗及预防提供科学、准确的实验室依据.Suspension array technology,a bead-based multiplex assay,allows a fast and automatic detection of multi-analytes and batch samples in limited sample volume,and it is great potential clinical application in the determination of autoimmune antibodies,muhitumor markers,the phenotype of Human Leukocyte Antigen and the genes of infectious pathogens.However,currently,there are various limitations which hinder the clinical application of this technology broadly.The globally accepted panel of multi analytes,performance criteria,and quality control programs should be established before we get benefit from this high throughput,sensitivity and repeatability platform,which will help to provide scientific and accurate laboratory data for the clinical diagnosis,treatment and prevention.

关 键 词：微球体 流式细胞术 微阵列分析 高通量核苷酸测序 

分 类 号：R446[医药卫生—诊断学]