ERK在黑素瘤细胞和人正常黑色素细胞中的表达  

Expression of ERK in Melanophores and Melanoma Cells

作　　者：张雪英[1] 陈官芝[2] 潘敏[2] 

机构地区：[1]山东省单县中心医院,山东菏泽274399 [2]青岛大学医学院附属医院,山东青岛266003

出　　处：《皮肤性病诊疗学杂志》2014年第4期287-290,共4页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：山东省自然科学基金项目(编号:ZR2010HM022)

摘　　要：目的:研究ERK1/2和P-ERK1/2在人正常黑色素细胞和黑素瘤细胞A375中表达强度的差异,并分析其意义。方法:从健康青年人阴茎包皮中分离并培养原代黑色素细胞;传代培养黑素瘤细胞A375;Western-blot检测两种细胞中ERK1/2和P-ERK1/2的表达强度,并进行统计比较。结果:ERK1/2、P-ERK1/2在黑素瘤细胞A375的表达强度均明显高于人正常黑色素细胞,差异均具有统计学意义(t值分别为17.75、104.39,P值均<0.01)。结论:黑素瘤的发生、发展可能与MAPK/ERK通路有关。Objective：To investigate the difference of expression intensity of ERK1/2 and P- ERK1/2 between melanophores and melanoma cell A375. Methods：Melanin ceils from the penis foreskin of normal young man were separated and primary cultured, and the subcultured melanoma cells A375 were adopted in the study. The expression of ERK1/2 and P-ERK1/2 in two cells were dected by western-blot, and the data have been analyzed on statistically. Results：The protein level of ERK1/2 in melanoma cells A375 was higher than that in melanophores （t = 17.75 ,P 〈 0. 01 ） , and the protein level of P-ERK1/2 in melanoma cells A375 was significantly higher than that in melanophores too（ t = 104. 39, P 〈 0. 01 ）. The differences were statistically significant. Condusion：The development of melanoma might be associated with MAPK/ERK pathway.

关 键 词：ERK1 2 P-ERK1 2 人正常黑色素细胞 黑素瘤细胞A375 

分 类 号：R739.5[医药卫生—肿瘤]