过表达少突胶质细胞转录因子2加速小鼠胚胎干细胞分化为神经元-胶质细胞抗原2阳性少突胶质祖细胞样细胞  

Oligodendrocyte transcription factor 2 overexpression facilitates the differentiation of mouse embryonic stem cells into neuron-glial antigen 2 positive oligodendrocyte progenitor-like cells

作　　者：曲学彬[1] 王贝[1] 罗梦娇[1] 郭瑞[1] 王会平[1] 张强[1] 于红丽[1] 姚瑞芹[1] 

机构地区：[1]徐州医学院生物学教研室,徐州221009

出　　处：《解剖学杂志》2014年第4期480-481,486,F0004,共4页Chinese Journal of Anatomy

基　　金：国家自然科学基金（81271345,81302510）;江苏省自然科学基金（BK20131132,BK20130221）

摘　　要：目的：观察少突胶质细胞转录因子2（O1ig2）过表达对小鼠胚胎干细胞（mESCs）向少突胶质祖细胞（OPCs）分化的影响.方法：将体外培养的mESCs分为空载体感染和Olig2-GFP慢病毒感染组,免疫荧光显色检测Olig2的表达.感染后9、14、21 d和28 d神经元-胶质细胞抗原2（NG2）免疫荧光显色观察mESCs向OPCs的分化情况.结果：Olig2-GFP慢病毒感染组mESCs经筛选后均为Olig2＋/GFP＋,而空载体感染组mESCs无Olig2表达.感染后9d,两组细胞均无NG2表达;感染后14d,Olig2-GFP慢病毒感染组NG2高表达,空载体感染组仅有少数细胞为NG2弱阳性;感染后21d与14 d相比,两组均无明显变化;感染后28d,两组细胞均有较强的NG2表达,组间无差异.结论：过表达Olig2能够加速mESCs分化为NG2＋的OPCs样细胞.Objective：To observe the effect of transcription factor Olig2 overexpression on the differentiation of mouse embryonic stem cells （mESCs） into oligodendrocyte progenitor cells （OPCs）.Methods：The mESCs were assigned to empty vector lentiviral infected mESCs （vector group） and Olig2-GFP lentiviral infected mESCs （Olig2 group） to test the expression of Olig2 by immunofluorescence staining.On the 9 d,14 d,21 d and 28 d after infection,the expression of neuron-glial antigen 2 （NG2） was detected using immunofluorescence staining to show the differentiation of mESCs into OPCs.Results：mESCs of the Olig2 group were all Olig2＋/GFP＋ cells after selection,while mESCs of the vector group did not express Olig2.On 9 d after infection,mESCs of neither group expressed NG2.On 14 d,mESCs in Olig2 group expressed a high level of NG2,while there were only fewer NG2＋ cells in the vector group,which had no changes on 21 d.Until 28 d after infection,mESCs of both groups were all NG2＋ cells without difference between each other.Conclusion：Olig2 overexpression can facilitate the differentiation of mESCs into NG2＋ OPC like cells.

关 键 词：少突胶质细胞转录因子2 基因过表达 小鼠胚胎干细胞 少突胶质祖细胞 分化 

分 类 号：R743[医药卫生—神经病学与精神病学]